10 Questions 36 Answers 0 Followers
Questions related from Md Abdul Halim
I want to buy these compound. These are usually used to detect H2O2. I have checked sigma chemical directory also http://www.chemexper.com/index.shtml but could not find out. I would appreciate if...
12 December 2014 2,745 7 View
When first three chemicals (K2MnO4+HNO3+NaOH) were mixed with each other (200 µL H2O, 5 µL 10 M NaOH, 5 µL 0.01 N HNO3, 5µL 1 M K2MnO4) there was no color change. But when I added sucralose (200...
11 November 2014 6,966 6 View
Sucralose is manufactured by the selective chlorination of sucrose (table sugar), which substitutes three of the hydroxyl groups with chlorine. Sucralose (a "chlorinated" carbohydrate) has some...
01 January 2014 7,618 0 View
I am working with human colonic smooth muscle cells. The goal is to see drug effects on intracellular calcium level.
12 December 2013 7,828 8 View
I am working with human colonic smooth muscle cells. We don´t have our own imaging system. So I need to transfer cell often from our lab to another lab (nearly 1 km distance). I am using styrofoam...
06 June 2013 618 11 View
I am developing an assay where we need to separate sucralose from urine. The problem is urine (control) gives peak at the same time. I have tried with phase partitioning but it did not work well....
02 February 2013 1,093 4 View
I have protocol for smooth muscle cell isolation from human colon. Now I am trying to isolate myenteric plexus from human colon tissue.
01 January 2013 6,181 6 View
I am trying set up some assay where I need to use endotoxin free plasma as control. Do you have any idea?
12 December 2012 7,952 3 View
I am trying to measure intracellular calcium in blood lymphocyte and colonic smooth muscle cells. Usually Lymphocytes don't attach on glass coverslip. So I am using poly-l-lysine to enhance...
11 November 2012 2,601 20 View
I am trying to culture human colonic smooth muscle cells. What I observed in the culture flask is a mixture of cells with tissue debris.
11 November 2012 2,601 2 View
