Md Abdul Halim

10 Questions 36 Answers 0 Followers

Questions related from Md Abdul Halim

Where can I buy naphthofluorescein disulfonyl ester, 2′,7′-dichlorofluorescein benzenesulfonic ester and 5-(4-cyanobenzoyl)carbonylfluorescein??

I want to buy these compound. These are usually used to detect H2O2. I have checked sigma chemical directory also http://www.chemexper.com/index.shtml but could not find out. I would appreciate if...

12 December 2014 2,733 7 View

What could be the reaction products of K2MnO4+HNO3+NaOH+Sucralose=?

When first three chemicals (K2MnO4+HNO3+NaOH) were mixed with each other (200 µL H2O, 5 µL 10 M NaOH, 5 µL 0.01 N HNO3, 5µL 1 M K2MnO4) there was no color change. But when I added sucralose (200...

11 November 2014 6,953 6 View

Is it possible to substitute chloride with iodide or Iodine-125 in sucralose?

Sucralose is manufactured by the selective chlorination of sucrose (table sugar), which substitutes three of the hydroxyl groups with chlorine. Sucralose (a "chlorinated" carbohydrate) has some...

01 January 2014 7,610 0 View

Is there any easy and low cost method to check smooth muscle cells viability?

I am working with human colonic smooth muscle cells. The goal is to see drug effects on intracellular calcium level.

12 December 2013 7,819 8 View

Can I transfer cell culture from place to place?

I am working with human colonic smooth muscle cells. We don´t have our own imaging system. So I need to transfer cell often from our lab to another lab (nearly 1 km distance). I am using styrofoam...

06 June 2013 610 11 View

How can I remove urobilin from urine?

I am developing an assay where we need to separate sucralose from urine. The problem is urine (control) gives peak at the same time. I have tried with phase partitioning but it did not work well....

02 February 2013 1,081 4 View

Need protocol for isolation of myenteric plexus from human colon tissue.

I have protocol for smooth muscle cell isolation from human colon. Now I am trying to isolate myenteric plexus from human colon tissue.

01 January 2013 6,169 6 View

How to prepare endotoxin free plasma sample?

I am trying set up some assay where I need to use endotoxin free plasma as control. Do you have any idea?

12 December 2012 7,938 3 View

How can I improve intracellular Calcium measurement in Lymphocytes and smooth muscle cells?

I am trying to measure intracellular calcium in blood lymphocyte and colonic smooth muscle cells. Usually Lymphocytes don't attach on glass coverslip. So I am using poly-l-lysine to enhance...

11 November 2012 2,588 20 View

Is it possible to separate colonic smooth muscle cells from fibroblast using gradient cetrifugation?

I am trying to culture human colonic smooth muscle cells. What I observed in the culture flask is a mixture of cells with tissue debris.

11 November 2012 2,587 2 View