I have protocol for smooth muscle cell isolation from human colon. Now I am trying to isolate myenteric plexus from human colon tissue.
Looks like this article* might be of help. It describes a fairly simple method of isolating myenteric plexus neuronal precursor cells from young human bowel samples. Depending on what you are trying to do, a multiple passage neurosphere assay might be your best bet for getting "pure" neuronal samples. You could also FACS sort your samples using neuronal markers.
*"Isolation and cultivation of neuronal precursor cells from the developing human enteric nervous system as a tool for cell therapy in dysganglionosis" Rauch et al
Looks like this article* might be of help. It describes a fairly simple method of isolating myenteric plexus neuronal precursor cells from young human bowel samples. Depending on what you are trying to do, a multiple passage neurosphere assay might be your best bet for getting "pure" neuronal samples. You could also FACS sort your samples using neuronal markers.
*"Isolation and cultivation of neuronal precursor cells from the developing human enteric nervous system as a tool for cell therapy in dysganglionosis" Rauch et al
Hi,
Thanks, they used human fetuses or young children like up to 5 yrs old to isolate myenteric plexus.
Is it possible to isolate myenteric plexus from adult human??
Our primary goal is to see neurotransmitter release.
It has been done in rats in the paper "Isolation of Enteric Ganglia from the Myenteric Plexus of Adult Rats" by Christine B. Jaeger. I bet the methods could easily be altered for use in humans.
Have you considered trying to culture the whole tissue? Maybe using slice culture techniques? That would maintain some of the in vivo connectivity with the convenience of ex vivo manipulations.
Hi,
Thanks for your help, I haven´t tried that. But I will try this next time.
You might follow the technique of Dr (now Prof) Simon Brookes when he was doing a post-doc in my research unit many years ago.
See:
Brookes SJH, Ewart WR, Wingate DL: Intracellular recordings from myenteric neurones in the human colon. J Physiol 1987, 390:305-331
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