The choice of solvent for dissolving secondary metabolites extracted from Aspergillus for use on MCF7 cells can depend on the solubility of the metabolites and the intended use. Here's a general guideline for preparing these compounds:

Solvent Selection

For dissolving secondary metabolites from Aspergillus for MCF7 cell anticancer studies, DMSO is the recommended solvent due to its superior dissolution properties and cell culture compatibility. Create a concentrated stock solution (typically 10 mM) by adding DMSO to the powdered metabolites, vortexing thoroughly, and using sonication or gentle heating if needed for complete dissolution. Centrifuge to remove any undissolved particles, filter-sterilize the supernatant, and store aliquots at -20°C or -80°C. When using in cell culture, dilute the stock in media immediately before use, ensuring the final DMSO concentration remains below 0.1% to minimize solvent effects on cells.