I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative. Thanks!
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
HI , i am concerned about any resist hardening effect of Post bake my LOR +PPR coated sample @90C for 5 mins. I wanted to do liftoff in acetone but the metals are hard to peel off. I cant use PG...
18 June 2024 5,741 0 View
Can we restrict the vertical and horizontal displacement in two directions for the lower semicircular in bottom hole of CT specimen and apply incremental displacement in the upper semicircular in...
09 May 2024 5,273 4 View
Hi fellow researchers, I have got a noob query regarding the question of the post. I am trying to build a data set of enzymes (from UniProt data base) that would contain protein sequences and...
21 April 2024 7,281 4 View
It is very well known that, owing to the soft lattice, and ionic bonds, metal halide perovskites shows easy movements of different ionic species under external perturbation. Due to this the...
28 March 2024 4,925 2 View
As we know, lymph nodes have a mixed population of cells, how do we ensure only B cells are getting isolated and subsequently fused with myeloma cells? Thanks in advance!
30 January 2024 3,449 2 View
What are the key considerations and steps involved in the simple centrifugation method for isolating lymphocytes withou using ready to use lymphocytes seperating medium?
29 January 2024 3,222 0 View
Is Low melting point agarose and agarose having low EEO the same? Is it possible to utilize agarose low EEO instead of low melting point agarose for COMET assay?
29 January 2024 6,834 2 View
Hi, I need help processing for big data. I am trying figure out ''How does Twitter process big data? Also, what can be done with big data?'' Can you explain briefly and/or can you suggest...
05 November 2023 9,122 3 View
have simulated a hydraulic jump. There is a difference in water and ambient(air)temperature and as such there is heat transfer across the water-air interface. I have used VOF model and Standard...
17 September 2023 5,277 1 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
AI-driven technologies offer previously unheard-of capabilities to process enormous volumes of data, extract insightful knowledge and improve predictive models, according to the UN’s World...
31 July 2024 9,733 3 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
What should be the zeta potential of bovine milk exosomes
25 July 2024 7,376 5 View
hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...
16 July 2024 9,044 10 View
In many experiments where HK-2 cells were stimulated with TGF-β at 10 ng/mL for 48 hours, I observed that even in the control group, the baseline expression level of fibrosis proteins like FN1 and...
15 July 2024 1,929 2 View
Hello everyone. I am working on my LV concentration porject, I use 500Kda hollowfiber to do it. The HCP and BSA removal rate is nearly 80%, but last time, I failed to run it. The HCP hasn't been...
11 July 2024 5,380 0 View
Hello, So I have mouse brain tissue MS data from one of my predecessors and I have human serum data from the hospital that I collaborate with. We are trying to find markers for the diagnosis of...
11 July 2024 8,484 0 View
I seed my cell (KG1a )in opti-mem media for transfection (without lipofectamine as control ) and after 2 hours I add FBS 10 ul in each wells all my cells after 24h in control wells or with...
07 July 2024 407 0 View
Hi all, I would like to know if it is possible to use Bovine Serum Albumin (BSA) in my blocking solution for immunofluorescence muscle fiber typing. The primary antibodies' host is mouse. The...
07 July 2024 7,967 3 View