I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative. Thanks!
in the function rbingham(n,A) in R software how can I construct that A (symmetric) matrix?
24 February 2021 3,528 3 View
during MD simulation, the atoms are not moving as per required. The problem (as per my observation) lies in energy minimization technique. upon application of tensile strain in x or y direction,...
22 February 2021 380 5 View
I need to carry out molecular dynamics simulation on Aluminium alloy. For this i need the crystal structure that which could be further replicated to form a structure.
22 February 2021 1,410 1 View
Can we measure the bulk composition of biomolecules present within an algal sample directly by placing its powdered form on a FT-IR spectroscopy stage? Or does it require a particular kind of...
30 January 2021 2,303 4 View
I have determined CWSI using the theoretical approach of Jackson et al., (1982) based on canopy temperature measurement in chickpea. at the final stages of crop growth some of my values of CWSI...
28 October 2020 9,992 3 View
Hi everyone, I want to increase the biomass of my E. coli culture for better plasmid yield. Use of different strains of cells and media or any other suggestions are welcome. Thanks!
11 October 2020 6,400 3 View
Dear colleagues, It will be a great help if someone can provide me small pieces (1.5 cm * 1.5 cm * 1.5 cm) of cast Al 5024 alloy and Al 7068 alloy (with and/or without T6 heat treatment. Happy to...
31 July 2020 3,593 1 View
I run repeated measures ANOVA for an intervention study. I have 3 intervention groups and 3-time points. The output in SPSS showed that there is no significant main effect of time and no...
02 April 2020 2,328 7 View
For scanning tunneling microscopy it is generally proposed that that the apex of the tip consist of single atom. For a Pt-Ir tip prepared by mechanical cut the apex atom sometime be of Pt or that...
11 March 2020 4,746 3 View
For testing the effectiveness of group intervention, I would like to conduct ANCOVA. I have one experimental and two comparison interventions. However, my data is not normally distributed. Also, I...
20 November 2019 432 9 View
If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis
02 March 2021 7,670 3 View
Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...
28 February 2021 7,310 3 View
I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.
28 February 2021 6,512 3 View
Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?
28 February 2021 3,653 4 View
Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...
27 February 2021 3,904 2 View
Hi everybody, Can anyone please suggest me an antibody anti-IgM Bovin conjugated Biotin ? I would like to use it in LUMINEX with a streptavidin conjugated phycoerythrin reporter. Thanks in...
25 February 2021 9,659 2 View
We have a old RD-ES cell line which is grow attached, but we got another vial of RD-ES which is very easy to get detached and grow in sphere in the medium. Do you know which morphology is normal...
24 February 2021 5,183 1 View
In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?
24 February 2021 7,064 3 View
To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...
23 February 2021 7,326 5 View
Could anyone suggest me the best procedure to measure the circulating estrogen levels in mice ? I came across the general methods where it can be measured in serum or urine but a detail suggestion...
22 February 2021 4,352 2 View