Hi ResearchGate community,

I am interested in using chemical flocculation to concentrate water samples for eDNA analysis. This is basically to avoid having to filter water.

I have come across interesting papers on this subject, however, people use chemical flocculation for the precipitation of entire cells (bacterial communities). I am wondering if this method can also work when we are dealing with environmental samples (cells + mitochondria + free floating extracellular DNA + mucous + fecal matter, etc). I would appreciate if anyone with experience with this could comment. I am interested in knowing what is the % recovery of this method.

Thanks