I am working on an orchid species. I have detected endoreduplication in the case of the mother plant and in in-vitro raised plants. Is that ok? Then, how do I measure 2C value and ploidy in that case?
Hi, Biswajt,
did you make a map or reduplications events?
Yes, endoreduplications can occurred in some cell type when cell go to perform specific function. Usually, it may be a outer cell layers in the root, when cell became much bigger in size to compare with inner layers, or in the leaf when cell size is essentail for it function. You can use flow cytometry with addition of the some stardant for detection of DNA stainability in certain organs.
Respected Sir,
yes, I performed flow cytometry with stems, leaves of plants from the nature and same from the tissue culture raised plants and that too with PLBS...So, is it species specific or organ specific or something else?
Sir, what is the consequence of this event i mean to say are there any significance of endoreduplication?
Hi, Biswajt, endoreduplication is organ specific and dependent from growth conditions, You can try to made endoreduplication map in your plants and you will see what excatly occurred in your growth conditions. Endoreduplications may have some significance during organ formation in some species, when the big differences in cell size between different cell layers should be achive. Endoreduplication is one of the possible tool for this. For the endoreduplication map you can use EdU/BrDU labelling, Good luck!
Sir i just want to check the ploidy level of my study plants and it just came out. I took the in vitro young leaves, stems and mature leaves of that orchid species......i found the same pattern...
Hi, Biswajt, so, already have an answer, old leaf (expanding one) have more cells with 4C and higher DNA contents,right?
do you have some standard for 2C? You can try to use nucleus from the meristemic cell whcih are in G1/G2 stage of the cell cycle. Good luck!
Sir,
Ya. I have Pisum sativum Citrad standard plants. I did analyze with Pisum leaf also at a same time with mother plants, in vitro raised plants.
I have been dealing with similar problems. Of course it depends on your research focus, but in most cases young leaves are best for ploidy determination...
ok...that means you are also getting same pattern.....I am getting the same pattern in case of donar plant(one year leaf), in vitro raised plants ( young expanding leaves) and PLBs also....so, what can I say...endoreduplication is species specific or tissue specific or what? Mam, can you please explain.
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