Flow Cytometry

Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell... | Contact experts in Flow Cytometry to get answers

1,363 views 2,162 posts

Questions related to Flow Cytometry

How do I successfully create/input this equation y=3.6844x + 0.002 in GraphPad prism to plot standard curves?

This is an equation (y=3.6844x + 0.002) advised by a kit manufacturer to be used to plot linear standard curves. I am trying to plot this in GraphPad Prism.

04 August 2023 1,205 2 View

How to gate precisely the live cells stained with fixable viability stains for flow cytometery?

I have a question maybe looks naive. I want to stain my cells with fixable viability stains. If we look at the chemistry of these stains, even live cells, although dim, can be stained. For...

05 June 2023 9,138 2 View

Can anyone recommend a good positive control to evaluate early apoptosis by FACS using Annexin V/PI method on MDA-MB-231 and MCF7 cells?

I am looking for suitable positive control for early apoptosis detection to compensation by using Annexin-V + propidium iodide in FACS. If anyone has an experience with such a drug kindly assist...

08 May 2023 6,834 2 View

What is the maximum timeframe one can wait to transfect cells after seeding them?

We are trying to transfect human fibroblast that are typically slow growing and can take a little while to get up to 70-80% confluency. We do try to plate them at a specific density, but sometimes...

08 May 2023 1,674 2 View

Is there any specific protocol for isolating phages against gram positive bacteria?

08 May 2023 1,719 2 View

Why does my freeze-dried Collagen scaffold dissolves when rehydrating instead of becoming a hydrogel again?

Hello, I use Collagen type I along with a buffer solution (consisting of PBS, NaOH, and deionized water) to make a 3D hydrogel. I then freeze dry it until it becomes a 3D scaffold, and seed cells...

16 April 2023 3,167 0 View

Western blot HIF 1 alpha?

What is the meaning of the two bands of HIf 1 alpha in western blot? Why sometimes it appears like two bands? thanks!

23 March 2023 327 1 View

Alternatives to Matrigel for in vivo work?

Like many people, we're looking for an alternative to Matrigel for flank tumor models. We typically used the high concentration matrigel solution 50-100uL mixed 1:1 to a cellular solution. We're...

22 March 2023 1,301 3 View

How to distinguish Pseudomonas putida from other Pseudomonas species?

Is there any media or molecular method by which we can distinguish Pseudomonas putida from other Pseudomonas species

05 March 2023 4,504 1 View

Is the centrifugation time in Amicon ultra depending on the starting concentration?

I'm concentrating my AAV preparation using Amicon Ultra 100KDa and I observed that sometimes I need really long time to concentrate. The point is that I have the longest centrifugation times when...

20 February 2023 653 2 View

What do two lines in FSC-A and FSC-H plot indicate in flow cytometry?

I am studying lymphocytes in the spleen using flow cytometry and I am trying to discriminate doublets in the FSC-A and FSC-H plot. In my data, there are two lines gated as P1 and P2. How should I...

19 February 2023 6,259 7 View

What are those red spots in the image when staining cells with trypan blue?

In the picture derived from light microscopy, there are some red stains apart from the blue ones. I am wondering, what could it be?

06 February 2023 3,589 0 View

What is the difference between Taqman Universal master mix and Taqman gene expression mastermix ?

I have FACS sorted some different types of immune cells (Innate lymphoid cells, gamma delta T cells, Natural killer T cells and Natural killer cells). As the number of cells were less in number, I...

22 January 2023 3,714 0 View

What is the best promoter for gene expression in Thp-1 cells?

Has anyone compared exogenous gene expression in Thp-1 cells using different promoters? Would EF-1 or CMV be better? Thanks!

19 January 2023 435 3 View

How to prepare Embryoid Bodies for Flow Cytometry?

Hello! It's me and Embryoid Bodies again. When it comes to total protein or mRNA isolation from EBs it's fine. But I have to admit that BCA shows low amount of total protein after lysis... But it...

10 January 2023 3,948 2 View

Is there good protocol for OVA peptide-pulsed dendritic cells in vitro?

Hi, I would like to know how to generate OVA peptide-pulsed dendritic cells in vitro. Dendritic cell would be co-culture with OT-II T cells, and measured T cell proliferation. 1. How long do I...

05 January 2023 3,825 1 View

About Block chain in agriculture?

Actually, I want to start research in agriculture. Whether blockchain or edge computing, or quantum com putting is good? I checked something about quantum computing. It seems still quantum...

11 December 2022 8,280 7 View

What are the best surface markers that could be used to seperate Th17 cells from peripheral blood of humans by Flow Cytometry?

Need to isolate or quantify Th17 cells from peripheral blood by flow cytometry.

10 December 2022 1,854 4 View

Basic flow cytometry question: how to perform counts for blood and platelet rich plasma?

If I prepare a stock of undiluted platelet rich plasma from whole blood, I then would like to perform a count on a flow cytometer. I take a small sample of e.g. 20uL and flow cytometer tells me...

30 November 2022 3,346 2 View

Does 1 ppm of an element in soil means 1 mg/Kg of the same element in soil?

I'm doing an elemental analysis of soils using an XRF Analyzer, the results obtained are in ppm. My question is, can I directly convert values from ppm to mg/Kg of soil?

10 November 2022 9,891 5 View

Why did I observe different cell counts when using CountBright™ Absolute Counting Beads for flow cytometry?

Dear reserchers, I performed "Flow cytometry" for T cells count using CD3 antibody (APC) and CountBright™ Absolute Counting Beads, for flow cytometry (C36950, Invitrogen). But, different cell...

09 November 2022 3,318 0 View

How did scientists discover which peak in FTIR spectroscopy belongs to each group in molecules?

Hello, anybody ever thought about how did scientists discover which peak belongs to each group in molecules?

08 November 2022 6,163 3 View

Mycobacteriophage plaque assay procedure?

Hello, I have a question about double layer agar method for plaque assay for mycobacterium. Why my bacterial lawn of Mycobacterium smegmatis is not homogeneous? What could be the reason of growth...

03 November 2022 7,892 0 View

How do I store mouse embryos (intended for explant culture) during transportation?

I have no nearby mouse facility and cannot bring live mice to my research facility. I therefore have to transport either the euthanized pregnant mouse or the mouse embryos (staged E11.5-E14.5)...

27 October 2022 9,884 6 View

Badges
Science method