Flow Cytometry

Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell... | Contact experts in Flow Cytometry to get answers

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Questions related to Flow Cytometry

Is an exam effective for students who haven't practiced for it?

Practice makes perfect. While it's difficult to expect good results on the exam without practice, it can still serve as a diagnostic tool. What are your thoughts?

12 April 2024 9,043 1 View

Would you mention some mandatory tests to study on moisture absorption of hydrogels?

Chitosan based hydrogel for moisture absorption and fertilizer releasing experiments.

24 March 2024 4,484 1 View

Plant DNA extraction in the MagNa Pure 96 Roche system?

Dear all, I hope you are well! I would like to know if anyone has used Roche's MagNa Pure 96 system to work with plant extraction? Can anyone share a pre-treatment?

04 March 2024 5,766 0 View

What statistical technique could I use to analyze the cytotoxicity of a treatment in mice PBMCs?

Hello! I was working with mice primary cell cultures to evaluate in vitro cytotoxicity of a treatment, I have an n of 10. The problem is that I don't know what technique to use because when I try...

28 January 2024 6,173 1 View

How can i improve my biocompatible microbot concept??

my microbot has a hydrogel shell which is promising but what about using PZT are there any other alternatives? for a more non-toxic ones like PVDF but this is just a concept since many claims...

13 January 2024 4,237 0 View

What is the role of microorganisms in sustaining the fertility of the soil and role of microorganisms in maintenance of environment?

28 December 2023 7,510 0 View

Why is maxquant not recognizing uniprot database fasta file?

We also tried to identify peptides from the yeasts used during the fermentation process and for such we tried to apply the Saccharomyces sp. and other Saccharomyces protein databases from UniProt....

10 November 2023 5,828 1 View

Do cover crops increase soil organic matter and why is the carbon footprint important for sustainability?

29 October 2023 6,233 10 View

Is there a manual or handbook that can facilitate the identification of gastric biopsy tissue sections as to which stage of cancer it belongs?

Such as adenocarcinoma, gastritis, metastasis etc.?

20 October 2023 8,791 0 View

How are the environment and economy interrelated and relationship between sustainable development and economic growth?

18 October 2023 2,318 5 View

How does Artificial Intelligence affect the agriculture sector and areas where robots can be used in agriculture?

16 October 2023 4,061 6 View

How do you get information about the number of chromosomes or DNA content?

I need to get information about the number of chromosomes or DNA content. I have seedlings from in vitro that were frozen at -20 degrees after liquidation of the culture. They are seedlings from...

29 September 2023 6,336 2 View

How do I successfully create/input this equation y=3.6844x + 0.002 in GraphPad prism to plot standard curves?

This is an equation (y=3.6844x + 0.002) advised by a kit manufacturer to be used to plot linear standard curves. I am trying to plot this in GraphPad Prism.

04 August 2023 1,218 2 View

How to calculate the volume fractions of pores in the metal samples?

How to calculate the volume fractions of pores in the alloy's samples?

09 July 2023 4,229 1 View

How to gate precisely the live cells stained with fixable viability stains for flow cytometery?

I have a question maybe looks naive. I want to stain my cells with fixable viability stains. If we look at the chemistry of these stains, even live cells, although dim, can be stained. For...

05 June 2023 9,154 2 View

How do I obtain an extraction of dog Brain Atlas Stereotaxic Maps for Saggital and Coronal Planes?

We want to conduct brain stereotaxic injection research in dogs, and we need a dog Brain Atlas Stereotaxic Maps similar to mouse brain stereotaxic injection.Any appropriate resources or channels...

05 June 2023 2,374 2 View

The percentage of different subsets of CD8+ T memory cells after influenza infection?

Hello, I am working on a project, in which we infect the mice with influenza virus and then we treat the mice with our compound or drug. and then we isolate the spleen of mice after 10 days to...

30 May 2023 6,451 0 View

Can anyone recommend a good positive control to evaluate early apoptosis by FACS using Annexin V/PI method on MDA-MB-231 and MCF7 cells?

I am looking for suitable positive control for early apoptosis detection to compensation by using Annexin-V + propidium iodide in FACS. If anyone has an experience with such a drug kindly assist...

08 May 2023 6,844 2 View

What is the maximum timeframe one can wait to transfect cells after seeding them?

We are trying to transfect human fibroblast that are typically slow growing and can take a little while to get up to 70-80% confluency. We do try to plate them at a specific density, but sometimes...

08 May 2023 1,684 2 View

Is there any specific protocol for isolating phages against gram positive bacteria?

08 May 2023 1,734 2 View

Colleagues, please tell me if it is possible to use outbred ICR/CD1 mice as laboratory animals when studying the effectiveness of MSC and why?

To conduct in vivo studies to evaluate the effectiveness of cell therapy

20 April 2023 8,463 1 View

Why does my freeze-dried Collagen scaffold dissolves when rehydrating instead of becoming a hydrogel again?

Hello, I use Collagen type I along with a buffer solution (consisting of PBS, NaOH, and deionized water) to make a 3D hydrogel. I then freeze dry it until it becomes a 3D scaffold, and seed cells...

16 April 2023 3,179 0 View

Western blot HIF 1 alpha?

What is the meaning of the two bands of HIf 1 alpha in western blot? Why sometimes it appears like two bands? thanks!

23 March 2023 341 1 View

In plants, I see Sigma M2 anti-FLAG and Invitrogen anti-FLAG non-specificity. Has anyone in tomato or maize seen these bands?

I expect a protein at 206kDa. The band seems to be in both wild-type and transgenic samples for tomato and maize . I was wondering if this size is just not documented as a common non-specific...

23 March 2023 4,964 0 View

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