Hi everybody :
Please : Can anybody tell me how long can I store the PCR primer solution under 20C ?
Are there any additives or methods which can improve the primer stability during the storage ?
Hi
Make some aliquots of your primers and store in -20C.
The main thing for stability is avoiding freeze thawing. Once you make your aliquots, leave your stock frozen and it stays for more than 5 years.
Hi
Make some aliquots of your primers and store in -20C.
The main thing for stability is avoiding freeze thawing. Once you make your aliquots, leave your stock frozen and it stays for more than 5 years.
Hi,
I had few µL of aliqots at -20°C (in H20 milli Q).
I try to used them 2 years after and it was ok.
As long as you store them in -20 and avoid thawing they can stay for years.
good luck
Avoid thawing. Stock prepare in TE will stay for years. Prepare working solution in DW.
only freeze thaw your working aliqotes, keep your primer stock (dissolved in TE) always at -20 degree freezer as long you can.
Best..
Stock primer should be prepared using TE buffer rather than a MilliQ.
TE buffer makeup primers will be more stable than milliq made primer stock solution.
Make some aliquots of your primers and store in -20C.
The main thing for stability is avoiding freeze thawing.
Once you make your aliquots, leave your stock frozen and it stays for more than 5 years if the freezing condition is maintained all the time.
thanks
Hi Hayder,
In our lab, we usually store the 100pmol stock at -80 and make some working aliquots of 10pmol that are stored at -20. Avoid repeated freeze thaw cycles and your primers will be stable for many years
I have stored primers in Milli Q water at -20 for years and they were working perfectly.
As mentioned above , I also store stock primers in TE at -20 for years with no problems. Working concentration primers are diluted in DW in aliquouts of 50ul or 100ul depending on your load work and stored at -20 as well. Good luck.
Make 100 ul aliquots of primer working stock in MilliQ and store them at -20 deg C. They should be fine even used a couple of times involving freeze thaw.
IDT has published a study on oligo stability. They found that oligos stored at -20°C remained intact for at least 24 months, regardless if they were stored in TE, nuclease-free water, or dry. Interestingly, oligos were even perfectly stable in TE at 37°C. Up to 30 freeze-thaw-cycles apparently do not impact oligo stability.
https://eu.idtdna.com/pages/education/decoded/article/storing-oligos-7-things-you-should-know
- Badges
- Science topic
- Similar topics
- Biological Science
- Biochemistry
- Biochemical Techniques