Hello researchers .
one of my colleagues develops a new drug and he think that this drug inhibit the activity of RNA polymerase II (transcription ) . the question is , how to estimate this inhibtion activty in cell line ?
best regards
Agree with @ Matthias R. Schaefer
measure mRNA transcription over time
Regards
I agree with my colleagues. Measure the mRNA transcription rate in relation with the drug dose to determine the minimum inhibitory concentration.
Regards.
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