Samra Sardar

9 Questions 42 Answers 0 Followers

Questions related from Samra Sardar

What is appropriate collagen induced arthritis (CIA) immunization protocol for DBA1/J mice?

I am planning to run a kinetic CIA experiment using male DBA1/J mice and I would like to see change in expression of gene of interest at various stages of disease. I have been reading about the...

04 April 2015 3,330 4 View

Anyone having experience with Genecopoeia All-in-One™ qPCR Primers for mouse transcripts?

I am wondering if anyone has used the pre-optimized primer pairs from Genecopoeia for RT-qPCR. I am considering these for some mouse transcripts.

02 February 2015 8,172 2 View

How to avoid saturation of positive control protein bands in Western blotting?

I am wondering how one can avoid saturation of loading controls while detecting low abundance protein of interest. I usually load 15-20microgram/lane of nuclear fraction from tissue homogenate to...

11 November 2014 1,024 5 View

Is CD3/CD28 stimulation and ConA stimulation activating T cells via different mechanisms?

I am trying to compare different groups of mice for T cell proliferation responses. I use unsorted splenocytes and stimulate them with different concentrations of antiCD3/CD28 antibodies and ConA....

10 October 2014 8,147 12 View

Is it possible to have a candidate gene with no coding SNPs but differential levels of methylation?

I am working on a genetic region (quantitative trait locus) that was identified in linkage analysis between two mouse strains that differed in response to induced arthritis. I am trying to...

10 October 2014 8,483 9 View

Could someone help with an in-vitro osteoclastogenesis assay protocol (from whole bone marrow culture)?

I'm planning to set-up an in-vitro osteoclastogenesis assay from mouse bone marrow culture, followed by TRAP staining. Could someone share a detailed, working protocol for that? Any suggestions...

07 July 2014 6,515 16 View

Any suggestions for a choice of growth and differentiation medium for bone-marrow stromal cells?

I am going to grow mouse BMSCs in-vitro and I want to differentiate them into osteoclasts. I am very new to this so I have been looking around for various protocols and publications for this kind...

04 April 2014 4,249 4 View

What is the importance of intronic SNPs in terms of structure/function of protein?

I am working with mouse models of RA and we have recently sequenced the whole genome of two strains to reveal genetic differences. Now I have a long list of SNPs for the 2Mbp region (relevant for...

03 March 2014 7,534 21 View

Nanodrop or BCA/Bradford assay?

I am interested to know if it should be okay to quantify proteins in a lysate by using nanodrop for purpose of Western Blotting. I have seen most of the protocols with either BCA or Bradford...

12 December 2013 1,731 7 View