How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

08 August 2024 1 5K Report

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample?

Thank you.

Yuri Mirgorod

Aiswariya K S

The fungus and all organic substances are decomposed by the high energy of the SEM rays. Therefore, you risk losing the sample. If you decide to do this, use a sample that you can lose for testing. To prepare, it is necessary to dry the sample from water, if it does not affect the shape of the fungus, in a desiccator over P2O5 and take a quick test shot. It is best to examine your sample in cryo-TEM. Then reviewers will have no questions about the experiment.

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