Hello every one
I'm working on micRNA expression and need the SNORD44 for normalization ,
please i need the sequences of SNORD44 primer because i cant find it in the published papers .
best regards
Assuming you are using stem-loop cDNA approach, the gold standard for miRNA qPCR. For the SNORD44 sequence at NR_002750.2 (NCBI),
1. Append "5' AGTCAG 3'" to the 3' end of your base stem-loop primer.
2. Pick forward primer below to match your universal reverse primer.
Forwards 5' to 3';
Seq Tm
CCTGGATGATGATAAGCAAATGCT 55
CCTGGATGATGATAAGCAAATGCTG 56
CCTGGATGATGATAAGCAAATGCTGA 57
CCTGGATGATGATAAGCAAATGCTGAC 58
CCTGGATGATGATAAGCAAATGCTGACT 59
CCTGGATGATGATAAGCAAATGCTGACTG 60
CCTGGATGATGATAAGCAAATGCTGACTGA 60
These are derived using my R helper tool at https://github.com/caanene1/miRNAprimer
You can also configure the source to match your need.
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