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Questions related from Risha Hazarika
I have a query. I have set a 60ul volume of reaction volume of PCR divided into 10 ul small volumes. After PCR is done , I find a reduction in volume from 10 ul to around 8 or 6 ul in some of...
03 March 2020 8,423 4 View
I isolated plasmid DNA by alkaline lysis method.I am getting a very weird graph in nanodrop. No peak was seen at 260 nm.Can anyone please tell what is the problem?
08 August 2019 6,651 4 View
I accidentally set the initial denaturation at 95 degress for 1 min, but It should have been for 5 mins. I realised it after I started the program while it had reached denaturation step at 95...
08 August 2019 7,645 6 View
I have 20mg/ml of RNAse A stock. I want to prepare 1mg/ml of volume 5 microlitre. How to proceed? In which solvent should RNAse A be dissolved; Double distilled water?
08 August 2019 3,962 2 View
I isolated pET28a plasmid and got the following bands as shown in picture . I want to know if I am getting correct bands and whether my plasmid is correct or not.Size of plasmid is 5369 bp but I...
07 July 2019 4,117 3 View
