What are the detailed buffer recipes and protocol to isolate and purify genomic DNA by silica spin column ?

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Mushtak T. S. Al-Ouqaili

You can have a look at this website,

https://www.thermofisher.com/iq/en/home/references/protocols/nucleic-acid-purification-and-analysis/dna-extraction-protocols/purification-of-genomic-dna.html

You can read those article online.

https://www.bioline.com/us/downloads/dl/file/id/873/isolate_ii_genomic_dna_kit_product_manual.pdf

Arizaldo Castro

You will need:

Lysis buffer

Tris buffer

Elution buffer

Wash buffer

Most spin column kits already have these buffers prepared. Are you planning to prepare them from scratch?

Hayder Obayes Hashim

Arizaldo Castro , yes i want to prepare all the buffer by my self .