How to extract DNA from Rovers Orcellex Brush?

Hello researchers I have a fresh oral tissue samples collected by Rovers Orcellex Brush , unfortunately I can not use any kit. Please, I need trusted protocol to extract DNA from this brush...

10 November 2019 8,270 3 View

What is the primers sequences of SNORD44 ?

Hello every one I'm working on micRNA expression and need the SNORD44 for normalization , please i need the sequences of SNORD44 primer because i cant find it in the published papers...

04 May 2019 4,751 1 View

What is the reasonable explanation of this abnormal haplotypes association odd ratio?

Hello everyone I'm working on haplotypes case control association study, for data analysis , I employed two online analysis softwares , The first is (SNPstats) which gave me extremely large odd...

11 December 2018 1,308 1 View

Why there are two different protocols to isolate DNA from frozen and fresh blood ?

Hello researchers all of us know, that we need a special protocol to extract DNA from frozen blood other than protocol which used to extract DNA from fresh blood . the question is : .what are...

08 September 2018 311 5 View

How to estimate RNA polymerase II activity in eukaryotes?

Hello researchers . one of my colleagues develops a new drug and he think that this drug inhibit the activity of RNA polymerase II (transcription ) . the question is , how to estimate this...

01 February 2018 7,574 2 View

Exonic or intronc region, which one is more potential to has polymorphisms that are associated with a trait ?

Hello researches I'm planning to sequence about 800 bp region of a certain gene to assess its association with a certain disease. the question is , do i have to choose an intronc or exonic...

31 December 2017 3,300 5 View

In HPLC/UV , can the negative peak resulted from fluorescent material?

Hello researchersthere are several reasons to observe a negative peak in HPLC/UV chromatogram , but can this negative peak be due to the presence of fluorescent material. best regards ....

08 September 2017 6,391 4 View

Annealing time or annealing temperature which one is better for PCR optimization ? and why?

Hello researchers the annealing temperature gradient is the routine step in PCR optimization , but why we do not use annealing time for optimization? regards .

07 August 2017 5,874 15 View

Is it essential to use high fidelity-pcr prior to sequencing ?

06 July 2017 3,748 9 View

What is the fate of proteins after they have been going out the SDS-PAGE gel ?

Hello researchers I just want to know what happen to the proteins molecules after they migrated out of the gel ( in SDS-PAGE) , of course the simple answer says that the protein migrated to the...

11 December 2016 5,839 11 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View