In HPLC/UV , can the negative peak resulted from fluorescent material?

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Bruce Neagle

Yes. Many times the fluorescent material is coloured, reacts with another compound, or is blanked against water and not a sample blank.

Andrea Carra

Do you have any wash process or valve operation prior to these peaks?

Dr Jannathulla R.

Yes, It is also one of the reason for obtaining negative peaks.

Hayder Obayes Hashim

thank you all for this feedback.