Dear Sarmita,

I have not worked with H9c2 cells but have performed hypertrophic stimulus for hiPSC-CMs and HL-1 cells.

I can suggest to test ISO or PE or a combination of both (100nM final concentration in the medium). The solution MUST BE prepared freshly and you should avoid exposure from light to get best results.

The medium at the time of treatment should contain serum.

For the cells I have treated, I achieved the best results after 48h or 72h treatment.

For hiPSC-CMs pre-starvation was not required but for HL-1 cells the pre-starvation step helped to achieve pronounced hypertrophy. So you would need to test this for your cells in particular.

You can find specific details of the hypertrophic stimulus in the online supplementary files from this publicationArticle Targeting muscle-enriched long non-coding RNA H19 reverses p...

Also apart from the above mentioned molecules you can also try out Cardiotrophin-1 to induce hypertrophy.

Hope this helps.

Best wishes

Shambhabi