What is your view point regarding the edible coating on the green leafy vegetables?

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How to define an anisotropic material with asymmetric elastic compliance/stiffness matrix in ANSYS APDL?

I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...

09 August 2024 5,048 2 View

I encountered a situation where the 16s PCR amplification worked perfectly with the DNA from the same sample, but using the same sample Don't working?

I isolated microbial DNA from marine water samples taken at various sites using the Qiagen Power Water DNA Isolation Kit, including a modified enzymatic lysis step. I encountered a situation where...

24 July 2024 9,688 0 View

During ws2 growth via cvd I have noticed black round spots on the si(100) substrate. please suggest me reason behind this?

I am using 20 mg of WO3, 800 mg of Sulphur, 925 oc growth temp, 280 oc S temp, and a 20 sccm gas flow rate.

05 May 2024 8,665 2 View

Can I allow cell phones in SPF animal house?

I have questions regarding the safe use of cell phones in animal research facilities. While I acknowledge the hygienic risk, we often spend a lot of time inside our barrier SPF facility and we...

14 April 2024 3,486 0 View

Inquiry Regarding Research on Female Sex Tourism in Jamaica?

Ladies and Gentleman, My name is Jana Maric, and I am a tourism management student at the university of applied sciences in Germany currently researching for my bachelor´s thesis in the field of...

08 April 2024 4,449 0 View

Can you find this relation ((β²+α²)/2αβ)sinh βb sin αa + cosh βb cos αa = cos k(a+b) from given determinant?

Please do it by hand, if not possible just do by some analytic engine.

26 March 2024 2,028 0 View

Why did I get the cracks on thin film after annealing? And how can I overcome this problem?

I deposited Ga2O3 thin film on fused quartz substrate at 500oc in Ar ambient by RF sputtering and then annealed at 900oc (very slow heating and very slow cooling i.e 1oc/min) in air ambient for 1...

11 February 2024 3,417 4 View

How to incorporate the negative slope (necking) part of an experimental tensile test data in ANSYS?

ANSYS multilinear material model does not support plastic strain vs stress data with a negative slope. Moreover, it assumes perfectly plastic (horizontal) behaviour from the end data point. How...

01 February 2024 9,950 0 View

Regarding UGC care list journal?

INDIAN JOURNAL OF NATURAL SCIENCES ISSN: 0976-0997 ( Print, Bimonthly ) Multidisciplinary Sciences Web of Science Indexes: Zoological Record is this journal UGC care list II category?

25 December 2023 4,017 2 View

Can anybody suggest some state-of-the-art research problems for Ph.D. on "AI in the academic sector"?

can anybody suggest some state-of-the-art research problems for Ph.D. on "AI in the academic sector"?

29 November 2023 3,419 4 View

Adhesion strength of coating?

How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...

10 August 2024 942 3 View

How do I conformally do PR spin coating on trench structure?

I did PR spin coating on trench structure. I used AZ P4620 PR and the thickness or PR is around 11um. The substrate is Si. And my trench structure depth is 141um(negative way). Even though I...

08 August 2024 2,298 0 View

Why do i have a weak plasma and no coating during rf sputtering?

I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.

07 August 2024 4,526 4 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Could dyes amplify the spectrum of light to a specific wavelength?

I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...

05 August 2024 3,290 1 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

What is the reason I get 400nm nanoparticles using an extruder with 200 nm membrane for cell membrane coating?

I am trying to coat my micelles and PLGA based nanoparticles with cell membrane using an Avanti mini extruder according to literature . I use 200nm membrane but my particles end up to be 400nm And...

30 July 2024 3,294 3 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View