What is your view point regarding the edible coating on the green leafy vegetables?

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Non motile black dots in animal cell culture, tightly adhered to the cells along the cell periphery. Is it a contamination? How to get rid of them?

I am seeing non-motile black dots of almost equal size along the periphery of skin melanoma cell line A375. They are present in almost all the cells. They don't turn the media turbid. However...

18 January 2021 5,151 1 View

Best flow cytometry markers for Thp1-derived M1 and M2 macrophages?

Hi everybody, I am trying to polarize and differentiate thp1 cells to M1 and M2 macrophages. There are lots of different protocols, so I've been trying different incubation times and...

02 November 2020 8,554 3 View

Have you ever seen two peaks after preparation small RNA library for NGS?

Hi, I have problem with my libraries, I use ClenTag Small RNA Library Preparation Kit by Trilink. The material is RNA from plasma cells. I have already done this two years ago and everythink...

25 August 2020 8,459 1 View

What are the key things to write and publish a paper in a good indexed journal?

The major spices and ingredients to add flavour in a paper for a journal in the domain of Computer Vision and Machine Learning.

27 June 2020 7,967 10 View

How to decide that a work is suitable for a conference or a journal in the field of Computer Science & Engineering?

What are the deciding factors to submit a paper in conference or journal.

21 June 2020 1,604 8 View

Whether Lambert Academic Publishing (LAP) provide printed copies to author? Do authors need paymet or sharing bank setails?

Hello Everyone !! Does anyone have any experience in publishing through Lambert Academic Publishing (LAP)? Recently I got a request mail from them regarding publication. I want to know about the...

25 April 2020 2,651 2 View

How can I write the .rpy and .jnl file in Abaqus cae for windows?

Hello :) I have the Abaqus CAE student version for Windows recently. My problem is: Abaqus does not write the .jnl and .rpy files automatically during a session as I am used to from the Linux...

30 March 2020 2,579 4 View

HSV1 in human tissue - dangerous after the fixation with PFA?

Hi, we are wondering about the HSV1 virus in human tissue. We want to perform in situ sequencing with this tissue, but we are not experienced with the virus-infected samples. Is it possible that...

14 October 2019 8,969 1 View

What details should be followed in MEGA-X for the Multiple Sequence Alignment of 16S data?

I have some 16S RNA sequence with which I want to do the phylogenetic analysis, so Initially I did BLAST against SILVA db and NACBI 16S db and went for MSA in MEGA-X using Muscle and Clustal both...

21 July 2019 9,320 3 View

While using R.E.D tools for charge calculation how to use Gaussian in the place of GAMESS? I have the file.p2n file and file.log ??

I have obtained the file.log after geometry optimisation done using GAUSSIAN and I do have the file.p2n after running Ante_RED-1.5.pl then I am getting an error of not having GAMESS while I wanna...

04 April 2019 4,228 1 View

Adherance of Polyaniline coating over stainless steel?

Is the electropolymerisation of polyaniline coating over stainless steel (SS) via Cyclic voltammetry technique is really adherant? Else how to improve the adhesion of the coating, whether any...

01 March 2021 8,220 2 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

How to immerse bricks in wax ( for coating ) ?

Do we immerse the bricks horizontally or vertically ? #bricks#waterproofingsurface#civil#engeenering#chemicalengineering#wax#parafinwax

28 February 2021 6,685 2 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

Why is my platinum counter electrode getting dirty during electrodeposition?

I am conducting anodic electrodeposition of MnO2 onto a stainless steel substrate, using a platinum strip as a counter electrode. This is done galvanostatically, with the current held between 5...

27 February 2021 1,829 3 View

What pH condition required to coat APTES on silica coated Fe3O4?

It's a hydrolysis reaction, which may produce by either acid or base-catalyzed reaction. so can anybody help me with this, which condition is suitable for this reaction?

26 February 2021 1,760 8 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View