Shambhabi Chatterjee

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Questions related from Shambhabi Chatterjee

Is mTeSR compatible with Geltrex?

Has anyone tried to culture iPSC on Geltrex with mTeSR ?? The data sheet suggest to use Matrigel or vitronectin for mTeSR but I would like to know if anyone has tried to use this medium with...

01 March 2017 337 5 View

PAd Delta F6 (AAV helper virus)- problems with plasmid prep

I am trying to do a routine maxiprep of a plasmid for AAV production. The plasmid is pAd Delta F6 (AAV helper virus from UPENN). I transformed into OneShot Stbl3 cells, plated on fresh LB agar...

27 May 2016 5,409 8 View

Can anyone help with a 3D object counter plugin of ImageJ?

I have two images of the same sample with different x,y,z dimensions. Can anyone explain - in order to quantify the same object - why do I need to enter larger number of voxels in the size filter...

19 June 2014 7,978 8 View