Shambhabi Chatterjee

6 Questions 112 Answers 0 Followers

Questions related from Shambhabi Chatterjee

Is there a marker for mitochondrial biogenesis ?

Does the ratio between mitochondrial fission vs fusion indicate mitochondrial biogenesis or is their a specific marker to anaylze mitochondrial biogenesis reliably? Looking forward to...

18 June 2018 9,542 4 View

Has anyone tried to seed hiPSC CM in Cell Culture Inserts?

I tried to coat the inserts ( Falcon™ Cell Culture Inserts ) but am unable to acheive decent survival of cells after seeding. These inserts are PET coated. The cells are able to attach well when...

28 March 2018 7,046 0 View

Is mTeSR compatible with Geltrex?

Has anyone tried to culture iPSC on Geltrex with mTeSR ?? The data sheet suggest to use Matrigel or vitronectin for mTeSR but I would like to know if anyone has tried to use this medium with...

01 March 2017 392 5 View

PAd Delta F6 (AAV helper virus)- problems with plasmid prep

I am trying to do a routine maxiprep of a plasmid for AAV production. The plasmid is pAd Delta F6 (AAV helper virus from UPENN). I transformed into OneShot Stbl3 cells, plated on fresh LB agar...

27 May 2016 5,469 8 View

Is it possible to remove the human beta-globin intron sequence from the pAAV-MCS ?

I want to clone in a gene which is above the packaging limit of the AAV..Is it possible to remove the human beta-globin intron sequence from the pAAV-MCS and replace it with the gene of...

28 April 2016 1,613 5 View

Can anyone help with a 3D object counter plugin of ImageJ?

I have two images of the same sample with different x,y,z dimensions. Can anyone explain - in order to quantify the same object - why do I need to enter larger number of voxels in the size filter...

19 June 2014 8,029 8 View