How to guide for in-silico miRNA analysis from TCGA for specific cancer ? Suggestion on bioinformatics guide or pipeline is expected
THx
I analyzed miRNA expression and got FDR correction of 0.99 between groups. The DE will be considered significant different only
08 September 2017 4,243 0 View
Can anyone suggest a good protocol to extract and enrich lncRNA after removing other RNA e.g. ribosomal, mRNA from serum sample ?
07 August 2017 3,591 1 View
How to use NormFinder software when there is undermined CT values for some samples? e.g. sample1 sample2 gene1 35 24 gene2 undeter 28 Thanks
07 August 2017 7,718 1 View
Is there a Standard protocol for agarose based 3D LNCap cell culture methods followed by protocol on clonogenic Assay ?
04 May 2017 4,338 2 View
HI How to plan further research after you find putative novel miRNAs ? Any suggestions on protocols or methodology for cancer biomarker discovery research ?
31 December 2016 9,688 0 View
Hi, Can anyone suggest good endogenous control RNU? for serum based exosomal miRNA ? I used RNU48, 4427975 TaqMan from thermofischer which did not work cT value above 35.
11 December 2016 9,879 4 View
How many samples are required for shotgun metogenomics data to have enough statistical inference ? e.g. 10 , 20 blood samples...
09 October 2016 8,105 3 View
hI, How to check if the bio-sample (e.g. Urine, Blood) is contaminated with human DNA ? Any suggestions about methods and protocol would be highly appreciated. Thanks
09 October 2016 8,332 1 View
What induces expression of AR-V7 in cell lines ?
08 September 2016 9,124 0 View
Hi In order to find RNA expression through RNAseq, what is the optimal number of samples are required ? Thanks
08 September 2016 2,816 0 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...
03 August 2024 5,828 4 View
I am looking for software to help analyze SELEX NGS data, including alignment, sequence enrichment, and other related tasks. Can anyone recommend suitable tools or software? Best wishes, Waleed
30 July 2024 1,061 5 View
PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...
28 July 2024 4,762 2 View
Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...
28 July 2024 6,938 1 View
The County health department is providing Breast cancer incident data. If colorectal cancer cases could be used as control, then we can get respective data from health department as well.
22 July 2024 9,954 4 View
Machine Learning for Automated Diagnosis of Skin Cancer with Dermoscopy Images
21 July 2024 5,471 0 View
Hello everyone, I work with polyacrylamide hydrogels, which I fictionalise with sulfo-sanpa and collagen I to then plate lung cancer cells. The problem is that I lose a significant amount of...
19 July 2024 3,906 0 View
To establish an orthotopic HCC model, C57BL/6 mice (male, 6-8 weeks) were orthotopically implanted with 3x106 Hepa1-6 cells (Over-expressed with a human gene) in 20uL Matrigel into the left lobe...
18 July 2024 9,177 2 View