How to guide for insilico miRNA analysis from TCGA ?

How to guide for in-silico miRNA analysis from TCGA for specific cancer ? Suggestion on bioinformatics guide or pipeline is expected THx

10 November 2017 8,511 0 View

Why FDR is close to 1 ?

I analyzed miRNA expression and got FDR correction of 0.99 between groups. The DE will be considered significant different only

08 September 2017 4,157 0 View

How to use NormFinder software when there is undermined CT values ?

07 August 2017 7,616 1 View

Suggestion on protocol for agarose based 3D LNCap cell culture ?

04 May 2017 4,223 2 View

Plan research on Novel miRNA ?

HI How to plan further research after you find putative novel miRNAs ? Any suggestions on protocols or methodology for cancer biomarker discovery research ?

31 December 2016 9,603 0 View

Which RNU works for serum based exosomal miRNA in qrt-pcr ?

11 December 2016 9,782 4 View

How many samples ?

How many samples are required for shotgun metogenomics data to have enough statistical inference ? e.g. 10 , 20 blood samples...

09 October 2016 7,990 3 View

How to check if the bio-sample is comtaminated with human DNA ?

hI, How to check if the bio-sample (e.g. Urine, Blood) is contaminated with human DNA ? Any suggestions about methods and protocol would be highly appreciated. Thanks

09 October 2016 8,235 1 View

What induces expression of AR-V7 ?

What induces expression of AR-V7 in cell lines ?

08 September 2016 9,013 0 View

How many sequencing is needed ?

Hi In order to find RNA expression through RNAseq, what is the optimal number of samples are required ? Thanks

08 September 2016 2,724 0 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View