Can anyone suggest a good protocol to extract lncRNA after removing mRNA from serum sample ?

08 August 2017 1 4K Report

Can anyone suggest a good protocol to extract and enrich lncRNA after removing other RNA e.g. ribosomal, mRNA from serum sample ?

Yoav Lubelsky

Since lncRNAs are biochemically identical to mRNAs you would have to enrich for specific sequences you look for sequencing.

http://www.rna-seqblog.com/quantitative-gene-profiling-of-long-noncoding-rnas-with-targeted-rna-sequencing/

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