How to guide for insilico miRNA analysis from TCGA ?

How to guide for in-silico miRNA analysis from TCGA for specific cancer ? Suggestion on bioinformatics guide or pipeline is expected THx

10 November 2017 8,511 0 View

Why FDR is close to 1 ?

I analyzed miRNA expression and got FDR correction of 0.99 between groups. The DE will be considered significant different only

08 September 2017 4,157 0 View

Can anyone suggest a good protocol to extract lncRNA after removing mRNA from serum sample ?

Can anyone suggest a good protocol to extract and enrich lncRNA after removing other RNA e.g. ribosomal, mRNA from serum sample ?

07 August 2017 3,500 1 View

How to use NormFinder software when there is undermined CT values ?

07 August 2017 7,616 1 View

Suggestion on protocol for agarose based 3D LNCap cell culture ?

04 May 2017 4,223 2 View

Plan research on Novel miRNA ?

HI How to plan further research after you find putative novel miRNAs ? Any suggestions on protocols or methodology for cancer biomarker discovery research ?

31 December 2016 9,603 0 View

Which RNU works for serum based exosomal miRNA in qrt-pcr ?

11 December 2016 9,782 4 View

How many samples ?

How many samples are required for shotgun metogenomics data to have enough statistical inference ? e.g. 10 , 20 blood samples...

09 October 2016 7,990 3 View

How to check if the bio-sample is comtaminated with human DNA ?

hI, How to check if the bio-sample (e.g. Urine, Blood) is contaminated with human DNA ? Any suggestions about methods and protocol would be highly appreciated. Thanks

09 October 2016 8,235 1 View

How many sequencing is needed ?

Hi In order to find RNA expression through RNAseq, what is the optimal number of samples are required ? Thanks

08 September 2016 2,724 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View