What induces expression of AR-V7 in cell lines ?
How to guide for in-silico miRNA analysis from TCGA for specific cancer ? Suggestion on bioinformatics guide or pipeline is expected THx
10 November 2017 8,598 0 View
I analyzed miRNA expression and got FDR correction of 0.99 between groups. The DE will be considered significant different only
08 September 2017 4,243 0 View
Can anyone suggest a good protocol to extract and enrich lncRNA after removing other RNA e.g. ribosomal, mRNA from serum sample ?
07 August 2017 3,591 1 View
How to use NormFinder software when there is undermined CT values for some samples? e.g. sample1 sample2 gene1 35 24 gene2 undeter 28 Thanks
07 August 2017 7,718 1 View
Is there a Standard protocol for agarose based 3D LNCap cell culture methods followed by protocol on clonogenic Assay ?
04 May 2017 4,338 2 View
HI How to plan further research after you find putative novel miRNAs ? Any suggestions on protocols or methodology for cancer biomarker discovery research ?
31 December 2016 9,688 0 View
Hi, Can anyone suggest good endogenous control RNU? for serum based exosomal miRNA ? I used RNU48, 4427975 TaqMan from thermofischer which did not work cT value above 35.
11 December 2016 9,879 4 View
How many samples are required for shotgun metogenomics data to have enough statistical inference ? e.g. 10 , 20 blood samples...
09 October 2016 8,105 3 View
hI, How to check if the bio-sample (e.g. Urine, Blood) is contaminated with human DNA ? Any suggestions about methods and protocol would be highly appreciated. Thanks
09 October 2016 8,332 1 View
Hi In order to find RNA expression through RNAseq, what is the optimal number of samples are required ? Thanks
08 September 2016 2,816 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
