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Questions related from Bjmal la
How to guide for in-silico miRNA analysis from TCGA for specific cancer ? Suggestion on bioinformatics guide or pipeline is expected THx
11 November 2017 8,573 0 View
I analyzed miRNA expression and got FDR correction of 0.99 between groups. The DE will be considered significant different only
09 September 2017 4,213 0 View
How to use NormFinder software when there is undermined CT values for some samples? e.g. sample1 sample2 gene1 35 24 gene2 undeter 28 Thanks
08 August 2017 7,688 1 View
Can anyone suggest a good protocol to extract and enrich lncRNA after removing other RNA e.g. ribosomal, mRNA from serum sample ?
08 August 2017 3,567 1 View
Is there a Standard protocol for agarose based 3D LNCap cell culture methods followed by protocol on clonogenic Assay ?
05 May 2017 4,310 2 View
HI How to plan further research after you find putative novel miRNAs ? Any suggestions on protocols or methodology for cancer biomarker discovery research ?
01 January 2017 9,658 0 View
Hi, Can anyone suggest good endogenous control RNU? for serum based exosomal miRNA ? I used RNU48, 4427975 TaqMan from thermofischer which did not work cT value above 35.
12 December 2016 9,850 4 View
hI, How to check if the bio-sample (e.g. Urine, Blood) is contaminated with human DNA ? Any suggestions about methods and protocol would be highly appreciated. Thanks
10 October 2016 8,302 1 View
How many samples are required for shotgun metogenomics data to have enough statistical inference ? e.g. 10 , 20 blood samples...
10 October 2016 8,062 3 View
What induces expression of AR-V7 in cell lines ?
09 September 2016 9,088 0 View
Hi In order to find RNA expression through RNAseq, what is the optimal number of samples are required ? Thanks
09 September 2016 2,789 0 View
HI, Which internal control is better for serum samples from prostate cancer patients for miRNA qrt-PCR profiling ?
05 May 2016 487 3 View
HiI used 1ml of serum sample and processed with a) total exosome isolation reagent; 4478360 b)Total exosome RNA isolation kit;4478545 and got hardly 5ng/ul of "mirna". Is this the normal...
04 April 2016 9,837 2 View
How to screen selected miRNAs from blood samples the easy way?
01 January 2016 9,538 3 View
Any suggestions on getting lyophilized exosome standards from serum that is suitable for prostate cancer research?
01 January 2016 7,596 4 View
