Bjmal la

15 Questions 15 Answers 0 Followers

Questions related from Bjmal la

How to guide for insilico miRNA analysis from TCGA ?

How to guide for in-silico miRNA analysis from TCGA for specific cancer ? Suggestion on bioinformatics guide or pipeline is expected THx

11 November 2017 8,564 0 View

Why FDR is close to 1 ?

I analyzed miRNA expression and got FDR correction of 0.99 between groups. The DE will be considered significant different only

09 September 2017 4,205 0 View

How to use NormFinder software when there is undermined CT values ?

How to use NormFinder software when there is undermined CT values for some samples? e.g. sample1 sample2 gene1 35 24 gene2 undeter 28 Thanks

08 August 2017 7,677 1 View

Can anyone suggest a good protocol to extract lncRNA after removing mRNA from serum sample ?

Can anyone suggest a good protocol to extract and enrich lncRNA after removing other RNA e.g. ribosomal, mRNA from serum sample ?

08 August 2017 3,557 1 View

Suggestion on protocol for agarose based 3D LNCap cell culture ?

Is there a Standard protocol for agarose based 3D LNCap cell culture methods followed by protocol on clonogenic Assay ?

05 May 2017 4,300 2 View

Plan research on Novel miRNA ?

HI How to plan further research after you find putative novel miRNAs ? Any suggestions on protocols or methodology for cancer biomarker discovery research ?

01 January 2017 9,649 0 View

Which RNU works for serum based exosomal miRNA in qrt-pcr ?

Hi, Can anyone suggest good endogenous control RNU? for serum based exosomal miRNA ? I used RNU48, 4427975 TaqMan from thermofischer which did not work cT value above 35.

12 December 2016 9,840 4 View

How to check if the bio-sample is comtaminated with human DNA ?

hI, How to check if the bio-sample (e.g. Urine, Blood) is contaminated with human DNA ? Any suggestions about methods and protocol would be highly appreciated. Thanks

10 October 2016 8,293 1 View

How many samples ?

How many samples are required for shotgun metogenomics data to have enough statistical inference ? e.g. 10 , 20 blood samples...

10 October 2016 8,050 3 View

What induces expression of AR-V7 ?

What induces expression of AR-V7 in cell lines ?

09 September 2016 9,079 0 View

How many sequencing is needed ?

Hi In order to find RNA expression through RNAseq, what is the optimal number of samples are required ? Thanks

09 September 2016 2,779 0 View

Which internal control is better ?

HI, Which internal control is better for serum samples from prostate cancer patients for miRNA qrt-PCR profiling ?

05 May 2016 481 3 View

Mirna from "exosomes" ?

HiI used 1ml of serum sample and processed with a) total exosome isolation reagent; 4478360 b)Total exosome RNA isolation kit;4478545 and got hardly 5ng/ul of "mirna". Is this the normal...

04 April 2016 9,827 2 View

How to screen selected miRNAs from blood samples the easy way?

01 January 2016 9,531 3 View

Lyophilized exosome standards?

Any suggestions on getting lyophilized exosome standards from serum that is suitable for prostate cancer research?

01 January 2016 7,582 4 View