How to guide for insilico miRNA analysis from TCGA ?

How to guide for in-silico miRNA analysis from TCGA for specific cancer ? Suggestion on bioinformatics guide or pipeline is expected THx

10 November 2017 8,511 0 View

Why FDR is close to 1 ?

I analyzed miRNA expression and got FDR correction of 0.99 between groups. The DE will be considered significant different only

08 September 2017 4,157 0 View

Can anyone suggest a good protocol to extract lncRNA after removing mRNA from serum sample ?

Can anyone suggest a good protocol to extract and enrich lncRNA after removing other RNA e.g. ribosomal, mRNA from serum sample ?

07 August 2017 3,500 1 View

Suggestion on protocol for agarose based 3D LNCap cell culture ?

04 May 2017 4,223 2 View

Plan research on Novel miRNA ?

HI How to plan further research after you find putative novel miRNAs ? Any suggestions on protocols or methodology for cancer biomarker discovery research ?

31 December 2016 9,603 0 View

Which RNU works for serum based exosomal miRNA in qrt-pcr ?

11 December 2016 9,782 4 View

How many samples ?

How many samples are required for shotgun metogenomics data to have enough statistical inference ? e.g. 10 , 20 blood samples...

09 October 2016 7,990 3 View

How to check if the bio-sample is comtaminated with human DNA ?

hI, How to check if the bio-sample (e.g. Urine, Blood) is contaminated with human DNA ? Any suggestions about methods and protocol would be highly appreciated. Thanks

09 October 2016 8,235 1 View

What induces expression of AR-V7 ?

What induces expression of AR-V7 in cell lines ?

08 September 2016 9,013 0 View

How many sequencing is needed ?

Hi In order to find RNA expression through RNAseq, what is the optimal number of samples are required ? Thanks

08 September 2016 2,724 0 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View