What should be the number of cells per well required for MTT assay (cell growth inhibition assay)?

What should be the optimal number of cells per well in a 96 well plate to perform cell growth inhibition assay or MTT assay? The cell line in question is the A-431 cell line. The cell line has a...

31 October 2019 1,763 4 View

Quantification of Asparagine using HPLC ?

I am trying to quantify asparagine in my fermentation broth using HPLC, C18 column with mobile phase 0.1% tfa: Acetonitrile (95:5 ). I'm not getting a perfect peak with the standard. The...

07 January 2018 3,050 5 View

Increase in pH with aeration in cell culture bioreactor?

09 September 2016 9,260 3 View

Have there been any attempt(s) of using AI(Artificial Intelligence) in predicting spatial patterns in geography?

In any domain whatsoever, urban, physiographical etc.. And if yes, what was the objective what were the consequencesany theory formulation if no, are there any current attempt going on ?scope of...

10 May 2016 9,529 4 View

How do I distinguish HE and EH type modes in exact analysis of multilayer circular waveguides?

When we study the exact mode analysis of circular waveguides (Ref. Light Transmission Optics by D. Marcuse and Fundamentals of optical waveguides by Okamoto), we get an eigen value equation. If we...

24 March 2015 8,072 3 View

How do I distinguish HE and EH type modes in exact analysis of multilayer circular waveguides?

When we study the exact mode analysis of circular waveguides (Ref. Light Transmission Optics by D. Marcuse and Fundamentals of optical waveguides by Okamoto), we get an eigen value equation. If we...

02 March 2015 1,694 4 View

Why did half of the capacitor charging energy go in parasitic resistance irrespective of resistance value ?

I know half of that energy is dissipated in heat in the resistance of the charging pathway, and only QVb/2 is finally stored on the capacitor at equilibrium I'm just going to lower the resistance...

14 February 2015 362 12 View

On what basis do we differentiate nanowires from nanorods?

Is it the aspect ratio (Diameter/Length), if yes then what is the range of value of Aspect ratio that defines nanowires and nanorods?

08 September 2014 8,609 15 View

What is the effect of infrared radiation on Coumarin 30 doped in PMMA?

Is it okay to use Coumarin 30 doped PMMA for fluorescence measurement over the spectrum from 500nm to 900nm? If not, then what are the limitations?

05 June 2014 5,350 0 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View