Can we do fatigue and creep fatigue interaction analysis in Abaqus Software?

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DOTA Quantification method?

Hi all!!! I have been trying to quantify the DOTA on my labelled protein but did not get any success. What method is best to quantify the DOTA labelling? If anyone has prior experience with...

16 January 2024 7,255 4 View

How can I improve the quality of a DSC (Differential Scanning Calorimetry) curve for journal publication when redrawing it?

The journal has provided a review point requesting an improvement in the quality of a diagram.

24 May 2023 928 0 View

Free cysteine in monoclonal antibody?

How to find out the available cysteine in the monoclonal antibody molecules. Is there any method other than Sulfhydryl assay using Ellman's reagent?

01 March 2023 3,009 4 View

How does the gene of interest get integrated in the genome?

We are using Freedom pCHO 1.0 vector to stably transfect the CHO cells. What is the mechanism of gene integration in the CHO cell? Is it homologous recombination or random integration? Please...

06 February 2022 1,632 0 View

How can I apply a boundary condition on a patched surface in Ansys Fluent?

Hi, I am trying to model a laser melting simulation, where a solid is being heated by a laser source (refereed as a Gaussian heat source). For that, I took a domain (Grey color in the attached...

18 February 2020 3,204 4 View

What should be the number of cells per well required for MTT assay (cell growth inhibition assay)?

What should be the optimal number of cells per well in a 96 well plate to perform cell growth inhibition assay or MTT assay? The cell line in question is the A-431 cell line. The cell line has a...

31 October 2019 1,862 4 View

Quantification of Asparagine using HPLC ?

I am trying to quantify asparagine in my fermentation broth using HPLC, C18 column with mobile phase 0.1% tfa: Acetonitrile (95:5 ). I'm not getting a perfect peak with the standard. The...

07 January 2018 3,130 5 View

How to remove formic acid from protein sample?

what is the procedure to remove formic acid from partially purified low molecular weight protein solution without using lyophilization?

30 August 2017 2,573 2 View

What are the bio assay for the quality check of monoclonal antibody?

What different types of bioassay are used for the quality check of a monoclonal antibody. How to check the quality of a biosimilar (monoclonal antibody) compared to its original molecule. The mAb...

16 March 2017 536 3 View

How can we identify the tryptophan oxidation site in mAbs?

I have tried with AAPH as a oxidizing agent for forceful oxidation of tryptophan but not able to get the desired oxidized peaks in LC. Can someone suggest the best way to do it?

06 March 2017 6,032 1 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

I have get this error when i calculated the geometrical optimization for prco3, it takes 12 hours until gives this message in the outputfile?

Fatal error in MPI_Allreduce: Other MPI error, error stack: MPI_Allreduce(1628)......: MPI_Allreduce(sbuf=000002459254A180, rbuf=000002459F86A140, count=4851, MPI_DOUBLE_COMPLEX, MPI_SUM,...

09 August 2024 7,615 1 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How to get moment output in Abaqus Standart?

I have input a moment load in module load Abaqus, i put my moment load on the node surface (using reference point). I have define moment in history output and make a set for moment too. But the...

08 August 2024 4,831 4 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to fix errors in my heat transfer steel structure with reinforced concrete slab model Abaqus?

I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...

07 August 2024 1,028 0 View