What should be the number of cells per well required for MTT assay (cell growth inhibition assay)?

What should be the optimal number of cells per well in a 96 well plate to perform cell growth inhibition assay or MTT assay? The cell line in question is the A-431 cell line. The cell line has a...

31 October 2019 1,763 4 View

Quantification of Asparagine using HPLC ?

I am trying to quantify asparagine in my fermentation broth using HPLC, C18 column with mobile phase 0.1% tfa: Acetonitrile (95:5 ). I'm not getting a perfect peak with the standard. The...

07 January 2018 3,050 5 View

Increase in pH with aeration in cell culture bioreactor?

09 September 2016 9,260 3 View

How to control gas flow for culturing CHO cell in reactor?

we want to maintain 5% co2 inside the fermentor, for that what condition are needed. eg what flow rate of co2 or o2 or air to be supplied. or anyone here used fermentor for mammalian cell culture...

26 June 2016 6,454 1 View

Have there been any attempt(s) of using AI(Artificial Intelligence) in predicting spatial patterns in geography?

In any domain whatsoever, urban, physiographical etc.. And if yes, what was the objective what were the consequencesany theory formulation if no, are there any current attempt going on ?scope of...

10 May 2016 9,529 4 View

How do I distinguish HE and EH type modes in exact analysis of multilayer circular waveguides?

When we study the exact mode analysis of circular waveguides (Ref. Light Transmission Optics by D. Marcuse and Fundamentals of optical waveguides by Okamoto), we get an eigen value equation. If we...

24 March 2015 8,072 3 View

How do I distinguish HE and EH type modes in exact analysis of multilayer circular waveguides?

When we study the exact mode analysis of circular waveguides (Ref. Light Transmission Optics by D. Marcuse and Fundamentals of optical waveguides by Okamoto), we get an eigen value equation. If we...

02 March 2015 1,694 4 View

Why did half of the capacitor charging energy go in parasitic resistance irrespective of resistance value ?

I know half of that energy is dissipated in heat in the resistance of the charging pathway, and only QVb/2 is finally stored on the capacitor at equilibrium I'm just going to lower the resistance...

14 February 2015 362 12 View

On what basis do we differentiate nanowires from nanorods?

Is it the aspect ratio (Diameter/Length), if yes then what is the range of value of Aspect ratio that defines nanowires and nanorods?

08 September 2014 8,609 15 View

What is the effect of infrared radiation on Coumarin 30 doped in PMMA?

Is it okay to use Coumarin 30 doped PMMA for fluorescence measurement over the spectrum from 500nm to 900nm? If not, then what are the limitations?

05 June 2014 5,350 0 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How to evaluate unsupervised clustering metrics when some elements do not have a cluster label in the result?

I run algorithms like HDBSCAN and MeanShift, and compare them with K-means and GaussianMixture. The first two algorithms do not return cluster labels for some elements in the dataset. My question...

27 February 2021 428 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View