What should be the number of cells per well required for MTT assay (cell growth inhibition assay)?

What should be the optimal number of cells per well in a 96 well plate to perform cell growth inhibition assay or MTT assay? The cell line in question is the A-431 cell line. The cell line has a...

31 October 2019 1,763 4 View

Quantification of Asparagine using HPLC ?

I am trying to quantify asparagine in my fermentation broth using HPLC, C18 column with mobile phase 0.1% tfa: Acetonitrile (95:5 ). I'm not getting a perfect peak with the standard. The...

07 January 2018 3,050 5 View

Increase in pH with aeration in cell culture bioreactor?

09 September 2016 9,260 3 View

How to control gas flow for culturing CHO cell in reactor?

we want to maintain 5% co2 inside the fermentor, for that what condition are needed. eg what flow rate of co2 or o2 or air to be supplied. or anyone here used fermentor for mammalian cell culture...

26 June 2016 6,454 1 View

Have there been any attempt(s) of using AI(Artificial Intelligence) in predicting spatial patterns in geography?

In any domain whatsoever, urban, physiographical etc.. And if yes, what was the objective what were the consequencesany theory formulation if no, are there any current attempt going on ?scope of...

10 May 2016 9,529 4 View

How do I distinguish HE and EH type modes in exact analysis of multilayer circular waveguides?

When we study the exact mode analysis of circular waveguides (Ref. Light Transmission Optics by D. Marcuse and Fundamentals of optical waveguides by Okamoto), we get an eigen value equation. If we...

24 March 2015 8,072 3 View

How do I distinguish HE and EH type modes in exact analysis of multilayer circular waveguides?

When we study the exact mode analysis of circular waveguides (Ref. Light Transmission Optics by D. Marcuse and Fundamentals of optical waveguides by Okamoto), we get an eigen value equation. If we...

02 March 2015 1,694 4 View

Why did half of the capacitor charging energy go in parasitic resistance irrespective of resistance value ?

I know half of that energy is dissipated in heat in the resistance of the charging pathway, and only QVb/2 is finally stored on the capacitor at equilibrium I'm just going to lower the resistance...

14 February 2015 362 12 View

On what basis do we differentiate nanowires from nanorods?

Is it the aspect ratio (Diameter/Length), if yes then what is the range of value of Aspect ratio that defines nanowires and nanorods?

08 September 2014 8,609 15 View

What is the effect of infrared radiation on Coumarin 30 doped in PMMA?

Is it okay to use Coumarin 30 doped PMMA for fluorescence measurement over the spectrum from 500nm to 900nm? If not, then what are the limitations?

05 June 2014 5,350 0 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View