How does the gene of interest get integrated in the genome?

07 February 2022 0 2K Report

We are using Freedom pCHO 1.0 vector to stably transfect the CHO cells. What is the mechanism of gene integration in the CHO cell? Is it homologous recombination or random integration? Please help. Thank you in advance.

More Jitender Nandal's questions See All

DOTA Quantification method?

Hi all!!! I have been trying to quantify the DOTA on my labelled protein but did not get any success. What method is best to quantify the DOTA labelling? If anyone has prior experience with...

16 January 2024 7,255 4 View

How can I improve the quality of a DSC (Differential Scanning Calorimetry) curve for journal publication when redrawing it?

The journal has provided a review point requesting an improvement in the quality of a diagram.

24 May 2023 928 0 View

Free cysteine in monoclonal antibody?

How to find out the available cysteine in the monoclonal antibody molecules. Is there any method other than Sulfhydryl assay using Ellman's reagent?

01 March 2023 3,009 4 View

Can we do fatigue and creep fatigue interaction analysis in Abaqus Software?

New material to be tested for fatigue creep and creep fatigue interaction on Abaqus Software.

29 March 2021 1,846 3 View

How can I apply a boundary condition on a patched surface in Ansys Fluent?

Hi, I am trying to model a laser melting simulation, where a solid is being heated by a laser source (refereed as a Gaussian heat source). For that, I took a domain (Grey color in the attached...

18 February 2020 3,204 4 View

What should be the number of cells per well required for MTT assay (cell growth inhibition assay)?

What should be the optimal number of cells per well in a 96 well plate to perform cell growth inhibition assay or MTT assay? The cell line in question is the A-431 cell line. The cell line has a...

31 October 2019 1,862 4 View

How to decide the bits per stage required for a pipelined ADC with last stage as folding ADC?

If i have to design 6-bit ADC, how can i decide the numbers of bits per stage.

27 January 2018 3,021 1 View

Quantification of Asparagine using HPLC ?

I am trying to quantify asparagine in my fermentation broth using HPLC, C18 column with mobile phase 0.1% tfa: Acetonitrile (95:5 ). I'm not getting a perfect peak with the standard. The...

07 January 2018 3,130 5 View

How to remove formic acid from protein sample?

what is the procedure to remove formic acid from partially purified low molecular weight protein solution without using lyophilization?

30 August 2017 2,573 2 View

What are the bio assay for the quality check of monoclonal antibody?

What different types of bioassay are used for the quality check of a monoclonal antibody. How to check the quality of a biosimilar (monoclonal antibody) compared to its original molecule. The mAb...

16 March 2017 536 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View

Best mask design for metal deposition aiming build a Perovskite solar cell device?

What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?

31 July 2024 4,835 3 View

How to culture and maintain Lx-2 cells?

Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...

31 July 2024 3,893 0 View

How to treat monolayer culture cell line with essential oil?

Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...

31 July 2024 5,301 2 View