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Questions related from Jitender Nandal
How to find out the available cysteine in the monoclonal antibody molecules. Is there any method other than Sulfhydryl assay using Ellman's reagent?
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We are using Freedom pCHO 1.0 vector to stably transfect the CHO cells. What is the mechanism of gene integration in the CHO cell? Is it homologous recombination or random integration? Please...
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What should be the optimal number of cells per well in a 96 well plate to perform cell growth inhibition assay or MTT assay? The cell line in question is the A-431 cell line. The cell line has a...
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I am trying to quantify asparagine in my fermentation broth using HPLC, C18 column with mobile phase 0.1% tfa: Acetonitrile (95:5 ). I'm not getting a perfect peak with the standard. The...
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what is the procedure to remove formic acid from partially purified low molecular weight protein solution without using lyophilization?
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How to decide or select a signal peptide for the overproduction of monoclonal antibody? what are the factors to be taken care of when selecting the signal peptide? answers with example will be of...
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Why there is an increase in pH with gassing in cell culture bioreactor? is it normal or there is some problem with the inlet gas? And how to overcome this problem. Thank You
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we want to maintain 5% co2 inside the fermentor, for that what condition are needed. eg what flow rate of co2 or o2 or air to be supplied. or anyone here used fermentor for mammalian cell culture...
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What are the best resources for finding antibody sequences? Is there any trusted database for this? where you people look for finding out the sequences you want to work on.
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What factors do you need before designing the exponential feeding in call culture? Also, what is the equation used in the exponential feeding? Experts in the group please comment. Thank you!!
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