12 Questions 15 Answers 0 Followers
Questions related from Jitender Nandal
Hi all!!! I have been trying to quantify the DOTA on my labelled protein but did not get any success. What method is best to quantify the DOTA labelling? If anyone has prior experience with...
17 January 2024 7,194 4 View
How to find out the available cysteine in the monoclonal antibody molecules. Is there any method other than Sulfhydryl assay using Ellman's reagent?
02 March 2023 2,981 4 View
We are using Freedom pCHO 1.0 vector to stably transfect the CHO cells. What is the mechanism of gene integration in the CHO cell? Is it homologous recombination or random integration? Please...
07 February 2022 1,612 0 View
What should be the optimal number of cells per well in a 96 well plate to perform cell growth inhibition assay or MTT assay? The cell line in question is the A-431 cell line. The cell line has a...
01 November 2019 1,837 4 View
I am trying to quantify asparagine in my fermentation broth using HPLC, C18 column with mobile phase 0.1% tfa: Acetonitrile (95:5 ). I'm not getting a perfect peak with the standard. The...
08 January 2018 3,098 5 View
what is the procedure to remove formic acid from partially purified low molecular weight protein solution without using lyophilization?
31 August 2017 2,547 2 View
What different types of bioassay are used for the quality check of a monoclonal antibody. How to check the quality of a biosimilar (monoclonal antibody) compared to its original molecule. The mAb...
17 March 2017 517 3 View
How to decide or select a signal peptide for the overproduction of monoclonal antibody? what are the factors to be taken care of when selecting the signal peptide? answers with example will be of...
12 January 2017 1,964 4 View
Why there is an increase in pH with gassing in cell culture bioreactor? is it normal or there is some problem with the inlet gas? And how to overcome this problem. Thank You
10 September 2016 9,313 3 View
we want to maintain 5% co2 inside the fermentor, for that what condition are needed. eg what flow rate of co2 or o2 or air to be supplied. or anyone here used fermentor for mammalian cell culture...
27 June 2016 6,516 1 View
What are the best resources for finding antibody sequences? Is there any trusted database for this? where you people look for finding out the sequences you want to work on.
01 January 1970 1,760 6 View
What factors do you need before designing the exponential feeding in call culture? Also, what is the equation used in the exponential feeding? Experts in the group please comment. Thank you!!
01 January 1970 9,243 1 View