What should be the number of cells per well required for MTT assay (cell growth inhibition assay)?

What should be the optimal number of cells per well in a 96 well plate to perform cell growth inhibition assay or MTT assay? The cell line in question is the A-431 cell line. The cell line has a...

31 October 2019 1,763 4 View

Quantification of Asparagine using HPLC ?

I am trying to quantify asparagine in my fermentation broth using HPLC, C18 column with mobile phase 0.1% tfa: Acetonitrile (95:5 ). I'm not getting a perfect peak with the standard. The...

07 January 2018 3,050 5 View

Increase in pH with aeration in cell culture bioreactor?

09 September 2016 9,260 3 View

How to control gas flow for culturing CHO cell in reactor?

we want to maintain 5% co2 inside the fermentor, for that what condition are needed. eg what flow rate of co2 or o2 or air to be supplied. or anyone here used fermentor for mammalian cell culture...

26 June 2016 6,454 1 View

Have there been any attempt(s) of using AI(Artificial Intelligence) in predicting spatial patterns in geography?

In any domain whatsoever, urban, physiographical etc.. And if yes, what was the objective what were the consequencesany theory formulation if no, are there any current attempt going on ?scope of...

10 May 2016 9,529 4 View

How do I distinguish HE and EH type modes in exact analysis of multilayer circular waveguides?

When we study the exact mode analysis of circular waveguides (Ref. Light Transmission Optics by D. Marcuse and Fundamentals of optical waveguides by Okamoto), we get an eigen value equation. If we...

24 March 2015 8,072 3 View

How do I distinguish HE and EH type modes in exact analysis of multilayer circular waveguides?

When we study the exact mode analysis of circular waveguides (Ref. Light Transmission Optics by D. Marcuse and Fundamentals of optical waveguides by Okamoto), we get an eigen value equation. If we...

02 March 2015 1,694 4 View

Why did half of the capacitor charging energy go in parasitic resistance irrespective of resistance value ?

I know half of that energy is dissipated in heat in the resistance of the charging pathway, and only QVb/2 is finally stored on the capacitor at equilibrium I'm just going to lower the resistance...

14 February 2015 362 12 View

On what basis do we differentiate nanowires from nanorods?

Is it the aspect ratio (Diameter/Length), if yes then what is the range of value of Aspect ratio that defines nanowires and nanorods?

08 September 2014 8,609 15 View

What is the effect of infrared radiation on Coumarin 30 doped in PMMA?

Is it okay to use Coumarin 30 doped PMMA for fluorescence measurement over the spectrum from 500nm to 900nm? If not, then what are the limitations?

05 June 2014 5,350 0 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

HPLC baseline failure (ACN/Water)?

My baseline increases without any explanation, it is only fixed by changing the solution B, will it be the column? Reagents? Bomb? Detector? Solution A: H3PO4 0.01% IN WATER Solution B: H3PO4...

21 February 2021 5,656 3 View