How much 0.2% Saponin should I add to lyse 1X 105 THP-1 derived macrophages per well in a 96 well TC plate?
Most of the papers referred to do not mention a volume or ratio.
Only one paper mentions a volume of 250ul 0.025% Saponin for 2 X 105 cells/ml plated in 24 well TC plate.
Article Spontaneously Occurring Small-Colony Variants of Staphylococ...