Can anyone recommend a method for ethylene gas detection in an air sample?

I am looking for a method to detect ethylene gas concentration in a green house. I have tried SPME , Head space and GC-MS analysis but results were not satisfied. So is it better to detect...

10 January 2019 7,832 3 View

Contamination in A549 cell culture?

I am culturing A549 cells, I have noticed there are white color particle like things floating on the media. it was visible to the naked eye also. there was no color change in the media or there...

22 February 2018 8,404 5 View

Why there were many floating cell, when THP-1 cells treated with PMA ?

I have done THP-1 cell differentiation into macrophages. I used RPMI as a media. and PMA 30nM concentration. after 24 hours incubation there were many floating cells. what could be the reason? and...

22 January 2018 3,473 3 View

Could anyone explain me the difference between non-verbal mental age and verbal mental age please?

14 March 2017 8,969 6 View

To do a DNA molecular dynamics simulation using GROMACS do I have to constraint the back bone?

When visualize my simulation using pymol, one strand is going out of the box while other strand is remaining inside. To do the simulation, do i have to constraint the backbone?

26 April 2016 5,329 3 View

How to get rid of the jumps in rmsd calculation in gromacs??

I used the command g_rms -f ../run01/run01.trr -s ../run01/run01.tpr -o rmsd01.xvg -fit rot+trans -pbc to calculate rmsd. But there are jumps in the molecule. Can someone please explain how to get...

29 March 2016 9,202 3 View

What should be the minimum qualifications to become a reviewer?

Having a MSc/ MPhil/ PhD? Number of publications?

20 June 2013 4,983 28 View

Global Tea Market and COVID-19

How Covid -19 impact on global tea market..? tea is considered as one of healthy drink for covid, is there any changes is market, drinking and purchasing pattern of tea in global market

01 January 1970 1,203 8 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

What is the best way to keep an anaerobic chamber anoxic in the absence of hydrogen?

I'm a graduate student who works with an anaerobe, so I often work in an anaerobic chamber. I've been working on an assay that involves the steady-state of the quinone/ quinol pool. Anoxic...

03 March 2021 2,971 1 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

How to indicate the degree of litter decomposed by microbes?

Any technique could detect the degree of litter decomposed by microbes? Or any indexes could do this job? I want to split the contribution of microbes during decomposition, so any suggestions...

23 February 2021 1,362 5 View

Can I get information about research laboratories that are currently having in culture, internationally/locally standardised microorganisms?

Microorganisms of interest include; E.coli; Salmonella typhimurium; Pseudomonas aeroginosa; Streptoccocus pneumoniae; Staphyloccocus aureus and Candida albicans. Locations closer to Port...

18 February 2021 6,500 3 View

How to measure pH in anaerobic digester?

I'm planning on doing research by using anaerobic digester with liquid substrate and want to see its pH changes periodically. Any suggestion on measuring the pH with maintaining its anaerobic...

09 February 2021 1,293 1 View

How do microbes tell the presence of an inorganic minaral in the soil?

Due to their ubiquitous nature, there is a tendency of the presence of microbes in a given mineral(s). For instance, if microorganisms that love the of pH less than 6 in the pH value, is isolated...

03 February 2021 4,630 10 View

How to lessen cell growth?

I am working on the 4T1 murine breast cancer cell line. from the first day I bought this cell line, the cell's growth rate is extremely high. I intend to make these cells resistant to a single...

24 January 2021 2,884 3 View