Thilini Abeyakone

5 Questions 6 Answers 0 Followers

Questions related from Thilini Abeyakone

How much 0.2% Saponin should I add to lyse THP-1 derived macrophages per well in a 96 well TC plate?

How much 0.2% Saponin should I add to lyse 1X 105 THP-1 derived macrophages per well in a 96 well TC plate? Most of the papers referred to do not mention a volume or ratio. Only one paper...

28 August 2022 627 0 View

How to prepare 0.2% Saponin for lysis of THP-1 derived macrophages?

I have planned to infect THP-1-derived macrophages with Mycobacterium tuberculosis. After infection, cells would be lysed to quantify intracellular viable bacteria. How should I prepare 0.2%...

26 August 2022 3,810 3 View

Half life of PMA intermediate dilutions?

For THP-1 differentiation we use reconstituted PMA at 1mg/ml. Our final concentration added to cells is 50ng/ml. Since final working concentration & volume required is low, we usually make...

04 March 2022 4,639 3 View

Which type of 96 well plate is suitable for intracellular growth assessment using Bactiter-Glo assay?

I am checking for intracellular growth of TB bacilli in macrophages in the presence and absence of certain antibiotics. The macrophages will be lysed and growth and viability detected using...

02 September 2021 5,220 2 View

How to get the trolox calibration curve in DPPH assay when there are two control absorbance values?

We have prepared a Trolox solution of 1mg/ml. From this 2 fold dilutions were prepared up to 31.25ug/ml. we have mixed 700ul of Trolox from each dilution with 700ul of 20ppm DPPH. from this 200ul...

27 September 2020 916 4 View