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Questions related from Thilini Abeyakone
How much 0.2% Saponin should I add to lyse 1X 105 THP-1 derived macrophages per well in a 96 well TC plate? Most of the papers referred to do not mention a volume or ratio. Only one paper...
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I have planned to infect THP-1-derived macrophages with Mycobacterium tuberculosis. After infection, cells would be lysed to quantify intracellular viable bacteria. How should I prepare 0.2%...
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For THP-1 differentiation we use reconstituted PMA at 1mg/ml. Our final concentration added to cells is 50ng/ml. Since final working concentration & volume required is low, we usually make...
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I am checking for intracellular growth of TB bacilli in macrophages in the presence and absence of certain antibiotics. The macrophages will be lysed and growth and viability detected using...
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