I have planned to infect THP-1-derived macrophages with Mycobacterium tuberculosis. After infection, cells would be lysed to quantify intracellular viable bacteria. How should I prepare 0.2% Saponin for cell lysis?
Weigh 200mg of saponin and dissolve in 100ml (1X) PBS or weigh 20mg of saponin and dissolve in 10ml (1X) PBS. Place the solution at 37 degree C until saponin has dissolved completely. Sterilize by filtration through a 0.22-µm filter. Store 0.2% saponin solution at 4 degree C. It is stable for months.