I am checking for intracellular growth of TB bacilli in macrophages in the presence and absence of certain antibiotics. The macrophages will be lysed and growth and viability detected using Bactiter-Glo assay in a 96 well plate format.
Could anyone tell me the type and brand of 96 well plates with a flat bottom, opaque walls that is suitable?
Does it need to be tissue culture-treated or do I need a high binding surface? Can I use a non-binding surface?
Hello Thilini Abeyakone
You can use tissue culture-treated, standard opaque walled, flat clear bottom 96- well plate. Use of white plates for luminescent assay will help.
I am attaching the following link for your reference. Please go through the brand of 96-well plate format which will be suitable for Bactiter-Glo assay.
https://ecatalog.corning.com/life-sciences/b2c/US/en/Microplates/Assay-Microplates/96-Well-Microplates/Corning%C2%AE-96-well-Black-Clear-and-White-Clear-Bottom-Polystyrene-Microplates/p/3610
Hope this helps.
Good Luck.
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