How can I obtain time series data by country on night-time light (brightness) ?

Hi everyone, Please, how can I obtain time series data by country on night-time light (brightness) ? I've tried, but so far all I can find are maps. I am looking for numerical data, especially in...

24 February 2021 8,625 4 View

Can one simply dry PCR products in air?

Hello all! Hope you are doing fine. For the needs of a library preparation (Illumina sequencing of PCR products basically), I need to dry 100ng of each PCR product and then reconstitute it in...

30 November 2020 8,246 11 View

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10 February 2020 7,162 5 View

Looking for research papers on Deep Learning - Neural Network used to delineate agricultural parcels based on satellite or UAV images?

Dears, I am looking for research papers leveraging Deep Neural Network to delineate field and parcel boundaries based on satellite or UAV images. I am especially interested in Sub-Saharan Africa...

18 February 2019 8,086 2 View

Which API could provide me MODIS NDVI in near real time (maximum 6 days delay) ?

20 January 2019 8,217 3 View

How to plot on a single graph multiple IRFs (Impluse Response Function) from different DSGE models on dynare?

Hi everyone, Please, I would like to know how with dynare I can get multiple IRFs (Impluse Response Function) from 02 different DSGE models (or mod. file) on the same graph. Could someone...

10 December 2018 2,934 3 View

How Can I find the Steady state of a DSGE model with dynare ?

Hi everyone, I hope you're all right! I am a Phd student at the end of my thesis writing. But I have a problem. In my last chapter, I would like to draw inspiration from the model developed by...

03 December 2018 8,975 2 View

Where can I find PAR (Photosynthetically Active Radiation) daily estimations dataset covering Uganda (mainland) from January 2017 till present?

Dears, I'm looking for a daily PAR dataset for Uganda mainland (from January 2017 till present times (the most recent, the best). I already investigated few sources unsuccessfully: ECMWF -...

26 October 2017 1,283 2 View

How to publish good and simple reports on medicinal plant research: The Asian Journal of Pharmacognosy

12 May 2017 9,991 15 View

Does anyone have good primers to test for mutations in mouse cancer cell lines: Kras, Braf, Pten, Pi3kca and Egfr mutation status?

Dear All, I'd like to characterize 2 mouse cell lines in terms of mutations and would like to know if you had good primers and protocol (or any other way to test it) to test the following...

23 April 2017 9,797 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View