Dear Colleagues what may be the identity of this fungi?

This pathogen has been isolated from wheat blast disease symptoms in Bangladesh. Isolate is cultured onto PDA media and two weeks age. I am confuse about its identity.Spores are septate Expecting...

02 March 2018 2,509 7 View

IAA extraction, purification and quantification protocol from rice seedling?

Please suggest me IAA extraction and determination protocol from rice.

02 March 2018 3,222 6 View

What is the putative identity of these fungal isolates?

Dear colleagues I have gotten these isolates from soil sample while trying to get entomopathogenic fungi. all are slow grower but produce numerous spores.

09 October 2017 8,206 11 View

Any biochemical test for Pyricularia?

To be sure, exlcuding PCR, is there any biochemical test or any kind of test for pyricularia? How spore production can be promoted for pyricularia? which media is good for maintenance and which...

04 May 2017 4,748 44 View

Anybody please suggest me the identity of this fungus?

04 May 2017 7,653 8 View

Which biochemical test I may perform to differentaite between Pseudomonas aeruginosa and Pseudomonas fluorescens?

I have few isolates that gave pigment on King's B. So which biochemical test I may do to be sure about P. fluorescens identity?

04 May 2017 6,580 24 View

Any selective media for isolating Pyricularia?

I used PDA and Swab cotton techniques to isolate Pyricularia but till now not successful. How may I start my job to isolate Pyricularia from rice sample?

04 May 2017 4,769 3 View

Identity of this fungi with clearer microscopic images, Neurospora fungi?

04 May 2017 3,573 15 View

How may I get Fusarium free phytophthora?

During Phytophthora culture I got fusarium contamination and its occurring every time. So which antibiotic or media or temperature I may use or maintain to get Fusarium free...

02 March 2017 8,823 16 View

Which factor control Beauveria performance in field level?

Dear all After application of Beauveria based insecticide which factors should be taken into consideration for better control of insects?

02 March 2017 3,642 1 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

What is the most suitable medium of ascomycetes fungi ??

What is the most suitable medium of ascomycetes fungi ??

26 February 2021 4,413 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

What are the LAB methodologies (procedures, equipment etc) for CO2 enrichment experiments to study crop responses?

I am doing a proposal for a research entitled 'GRAIN YIELD AND QUALITY RESPONSES OF WHEAT GENOTYPES UNDER CO2 ENRICHMENT AND EXTREME EVENTS' I will be using climate chamber and I would like to...

18 February 2021 6,494 4 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

Can one use just 250 micron sieve & still get a good number of mycorrhizal spores from the soil?

In a situation where one cannot get the appropriate & separate levels of sieve for mycorrhizal survey work, can just one level of sieve give good results & spore counts?

13 February 2021 3,760 2 View

Any specific reason, how sclerotium rolfsii can grow out of Petridish?

Is it related to nutrition or any other reason? Any other fungus have similar kind of growth pattern?

09 February 2021 4,357 4 View

Which fungicide are good for primary cells? Does it yeast contamination? How to get rid of them without killing the cells? How to get rid of spores?

Hello! Our lab is working with primary cells which we are isolating from the colorectal tissue. During the isolation, we are washing the tissue with pen-strep and then also add some pen-strep in...

07 February 2021 8,258 3 View