7 Questions 15 Answers 0 Followers
Questions related from Sehroon Khan
What could be the possible explanation that a gene is present in two parents but not expressed. However it is expressed in the F1hybrid. Does it have any connection with Ac/Ds transposition.
10 October 2017 2,426 0 View
Dear all Please send me a protocol to isolate endophytic bacillus species from potato, thanks.
03 March 2017 5,449 18 View
Dear All Could anyone help me guide for cloning and expression of heterologous protein gene in pP43NMK vector. As the pP43NMK has P43 (promotor), i need to insert SP on N-terminal of P43. So from...
09 September 2016 4,759 0 View
Hi Dear all Is there some other ways to desalt my crude protein sample, if i dont have dialysis tubes, The proteins are precipitated with 80% amonium salphate. I do not wanna to loss even 4kDa...
08 August 2016 5,252 7 View
Dear All If i have a gene sequence and i wanna to express it in E. coli, how will i know the sutaible signal peptide which could help in expressing the gene? The gene sequence was found through MS...
08 August 2016 6,622 8 View
Dear All I have three peptides sequences from MALDI TOF/TOF MS analysis, now i am confused where to blast in MASCOT database and the second confusion is that i have to separately blast each one or...
08 August 2016 4,149 6 View
i want to purify elicitor proteins from the crude proteins of cuture filterate of fungi, therefore, i need to precipitate (80% saturation) the crude proteins.
06 June 2016 4,050 1 View
