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Questions related from Mohammad Sharif Sarker
I have been isolated from wheat disease. Among several isolates this isolate produce these kind of spores.
04 April 2018 8,029 6 View
Please suggest me IAA extraction and determination protocol from rice.
03 March 2018 3,306 6 View
This pathogen has been isolated from wheat blast disease symptoms in Bangladesh. Isolate is cultured onto PDA media and two weeks age. I am confuse about its identity.Spores are septate Expecting...
03 March 2018 2,617 7 View
Dear colleagues I have gotten these isolates from soil sample while trying to get entomopathogenic fungi. all are slow grower but produce numerous spores.
10 October 2017 8,281 11 View
I have few isolates that gave pigment on King's B. So which biochemical test I may do to be sure about P. fluorescens identity?
05 May 2017 6,672 24 View
I got this fungi while I was trying to isolate Pyricularia. It produce orange color colony on PDA, Fast growing fungi, septate hypahe, spores are single celled.
05 May 2017 7,726 8 View
This fungi first time produce white hyphae on PDA but later it turns into orange color. I think its spores are orange color. Hyphae septate.
05 May 2017 3,646 15 View
I used PDA and Swab cotton techniques to isolate Pyricularia but till now not successful. How may I start my job to isolate Pyricularia from rice sample?
05 May 2017 4,876 3 View
To be sure, exlcuding PCR, is there any biochemical test or any kind of test for pyricularia? How spore production can be promoted for pyricularia? which media is good for maintenance and which...
05 May 2017 4,857 44 View
During Phytophthora culture I got fusarium contamination and its occurring every time. So which antibiotic or media or temperature I may use or maintain to get Fusarium free...
03 March 2017 8,919 16 View
Dear all After application of Beauveria based insecticide which factors should be taken into consideration for better control of insects?
03 March 2017 3,717 1 View
During rice experiment set with trichoderma and zinc solubilzing bacteria I used seedling dipping method. Now I want to apply second doses of trichoderma and zinc solubilizing bacteria but which...
03 March 2017 4,456 13 View
I used Chloramphenicol, Streptomycin, Gentamicin and Cefoxitin to remove bacteria from trichoderma spore. After sterilization microscopic observation showed bacateria free. but when i subcultured...
11 November 2016 2,272 2 View
In PDA Beauveria and other entomopathogen suspected isolates grow very slowly when I cultured. Which media I may use or which salt/carbon source I may use to get rapid growth and spore rapidly....
11 November 2016 8,520 20 View
For the first time I have isolated one Beauveria from dead bean aphid. so Now I want to isolate Metarhizium. But i am not sure what type of sample would be most suitable? is it soil or plant...
10 October 2016 9,608 5 View
this isolate got from rice. and whats identity? Regards Shuvrah
10 October 2016 6,150 7 View
Dear all which media is the best one for liquid culture of Beauveria? I have Beauveria isolates and now I want to apply it on insects to test its performance. first time i used nutrient broth and...
09 September 2016 5,339 2 View
for my research purpose now I need to isolate Pythium.I have seen few selective media for Pythium isolation but all of them are with rare antibiotics. So is there any simple way or selective media...
08 August 2016 3,795 1 View
Dear colleagues During dealing with chickpea rhizobium I have seen it produce only 4/5 noudle/plant. is it enough to meet up the nitrogen requirement of a single plant? I want to increase the...
08 August 2016 8,081 5 View
Agricultural biotechnology related research proposal how may I write? Any format?
08 August 2016 8,709 6 View
After completing PCR we stored all Tips, tissue papers, gloves etc in a storage box. Now its completely covered. So which method will be best to dispose it into the environment?
08 August 2016 9,818 4 View
I have seen three types of Christian- orthodox, catholic and roman Protestant. So what are reasons for this classification? What's the basic difference among this three?
08 August 2016 2,847 0 View
From my collection of Curvularia fungi isoaltes I want to check pathogenicity. Which crop/age of seedlings/techniques of inoculation I may use to get best result? Thanks Shuvrah Rehman
08 August 2016 9,620 3 View
while dealing with new isolation of soybean disease pathogen I got this fungi. But I dont know what's identity? I did not capture petri plate culture picture. If someone need I may share...
08 August 2016 2,796 10 View
I have been isolated this fungal isolate from country bean. Well i have attached colony pictures on PDA media and source from where I have gotten this pathogen. Hope it will help all of you. I...
01 January 1970 8,933 19 View
