Hey! I aim to generate a transgenic knockin zebrafish line that mimetizes a genetic condtition that leads to a certain disease on human. To do so, I need to insert a codon for mutagenic aminoacid into our gene of interest, however I was wondering that somehow I need a reporter to validate my transformation and follow up the disease onset/progression. Is it possible to insert both mutation in the middle of the gene and report sequence at the end at the same time? Or is it possible to insert the full cDNA of the modified gene fused to a reporter sequence and a stop codon before exon 1 of the native gene? I dont know the maximum size that cas9 allows to successfuly knock-in in zebrafish.