Farheen Shafique

3 Questions 14 Answers 0 Followers

Questions related from Farheen Shafique

Is there a risk of mutation after prepapring a cDNA or genomic library?

I was wondering, when we prepare genomic library how do we know that the sequence we cloned is wild and not got mutated during the process?

22 September 2022 9,815 6 View

Can I use the PCR primers as Sequence primers?

Hi there, I've amplified a beta globin gene in fragments using PCR primers. can I use same set of primers to sequence these fragments?

02 September 2021 2,514 3 View

Is there any inhibitors which could be used at low concentrations to delay G2 entry to increase the opportunity for in vivo gene insertion?

I am working on a disease model by using CRISPRs Cas9 and 12. I need a s/G2 phase inhibitor that could be used at low concentrations to delay G2 entry to increase the opportunity for gene insertion?

09 April 2019 925 0 View