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For in-vivo editing in model animals like zebrafish and mice, several brands of Cas9 protein are highly regarded. The Alt-R® S.p. Cas9 Nuclease from Integrated DNA Technologies (IDT) is known for its high efficiency and reliability. Another excellent option is the HiFi Cas9 Protein from IDT, which offers enhanced specificity and reduced off-target effects [1][2]. Additionally, the TrueCut™ Cas9 Protein v2 from Thermo Fisher Scientific is widely used for its robust performance in various experimental settings [3][4]. These proteins facilitate efficient gene editing by improving delivery and targeting within cells [5][6].
Reference
[1]Zhang, S., Shen, J., Li, D., & Cheng, Y. (2021). Strategies in the delivery of Cas9 ribonucleoprotein for CRISPR/Cas9 genome editing. Theranostics, 11, 614 - 648.
[2]Mout, R., Ray, M., Tonga, G. Y., Lee, Y., Tay, T., Sasaki, K., & Rotello, V. (2017). Direct Cytosolic Delivery of CRISPR/Cas9-Ribonucleoprotein for Efficient Gene Editing.. ACS nano, 11 3, 2452-2458 .
[3]Hu, P., Zhao, X., Zhang, Q., Li, W., & Zu, Y. (2018). Comparison of Various Nuclear Localization Signal-Fused Cas9 Proteins and Cas9 mRNA for Genome Editing in Zebrafish. G3: Genes|Genomes|Genetics, 8, 823 - 831.
[4]Dorner, L., Stratmann, B., Bader, L., Podobnik, M., & Irion, U. (2024). Efficient genome editing using modified Cas9 proteins in zebrafish.. Biology open, 13 4.
[5]Wang, D., Mou, H., Li, S., Li, Y., Hough, S. H., Tran, K., Li, J., Yin, H., Anderson, D. G., Sontheimer, E., Weng, Z., Gao, G., & Xue, W. (2015). Adenovirus-Mediated Somatic Genome Editing of Pten by CRISPR/Cas9 in Mouse Liver in Spite of Cas9-Specific Immune Responses.. Human gene therapy, 26 7, 432-42 .
[6]Qin, W., Kutny, P. M., Maser, R., Dion, S., Lamont, J. D., Zhang, Y., Perry, G. A., & Wang, H. (2016). Generating Mouse Models Using CRISPR‐Cas9‐Mediated Genome Editing. Current Protocols in Mouse Biology, 6, 39 - 66.
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