I have this question I really cant understand how will this work.
A polyclonal has a microRNA sequence targeting a gene that is involved in cell proliferation. How do you test the efficiency of knockdown of the expression using the microRNA.
Example: Artificial microRNAs (amiRNAs) have become an important tool to assess gene functions due to their high efficiency and specificity to decrease target gene expression. Based on the observed degree of complementarily between microRNAs (miRNAs) and their targets, it was widely accepted that plant miRNAs act at the mRNA stability level, while the animal miRNAs act at the translational level. Contrary to these canonical dogmas, recent evidence suggests that both plant and animal miRNAs act at both levels.
Hello Ellyn,
I would recomment using cell-lines, provided you know the sequence.
Warm regards,
Venk
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