I have to differenitate stem cells into preadipocyte and further into mature adipocyte. We want to quantify ECM produced by adipocytes. Not sure if I should use Immunoflurence or there is any other better method of achieving this goal?
After UVO treatment on PDMS, I got C=O stretch peak from ATR-FTIR. I used Sylgard184 (base:curing agent=100:4), and treated UVO 90 minutes on cured PDMS by UV ozone cleaner.
05 April 2024 1,902 0 View
I need to determine indentation and tissue stiffness. I already have paraffin embedded tissue, but not sure if that will give an accurate result or I should use a fresh tissue.
11 February 2024 2,115 0 View
I have to determine adipose tissue stiffness. My samples were preserved in NBF. Can I use those tissue sections? or should I use fresh tissue to identify tissue stiffness? Is there any protocol...
01 February 2024 7,883 0 View
I have to characterise fibroblasts and preadipocytes from fat tissue. MOst of the articles have used DMEM/ F12, while some have used DMEM. Is there any rational behind using specific media over...
21 November 2023 4,268 0 View
Examples of informal publishing: Blogs Podcasts Letters/Email YouTube videos Examples of formal publishing: Peer-reviewed journal articles Books printed by scholarly publishers Conference...
10 October 2023 3,434 0 View
I am sorting preadipocytes from SVF and now need to differentiate them into mature adipocytes. Does a mixed cocktail of preadipocyte differentiation media works best of making a cocktail by...
07 October 2023 1,142 0 View
Given the disproportionately high risk provided by blood-borne pathogens, regular monitoring of blood transfusions is required to avoid disease transmission. ELISA and Rapid diagnostic...
01 September 2023 7,864 1 View
As I know, there are 3 reference energy to find or compare band level when we need to band alignment data 1. fermi energy set to zero 2. Vacuum energy (from slab calculation) set to zero 3. Align...
03 August 2023 2,187 0 View
What's the hallmark of adipose tissue fibrosis? What techniques can be performed to identify and quantify adipose tissue fibrosis?
24 July 2023 2,705 0 View
I have to characterize fibroblast and myofibroblast in adipose tissue. Since these are connective tissue cells and ubiquitously present in all connective tissue therefore, isolation from a...
18 June 2023 1,307 1 View
I have been harvesting monocyte-derived macrophages from buffalo and cattle fresh whole blood using the same protocol for years and getting good macrophage cultures. Below is the protocol that I...
23 July 2024 4,073 2 View
Greetings all, My group has been trying to reproduce 3t3-L1 cell (preadipocytes) differentiation. The typical protocol involves 3 steps: the first step and second step both last for 2 days, and...
18 June 2024 2,728 1 View
I have a peculiar problem - in FBS or albumin containing media my cells (preadipocytes) are very easy to image (and therefore quantify using a combination of ilastik and CellProfiler). However,...
28 May 2024 3,780 0 View
I am using the hanging drop method to develop individual differentiated adipocyte spheroids from the stromal vascular fraction of adipose tissue. With our current timeline they end up 100-300um....
27 May 2024 1,303 1 View
I used regular freezing media (90% FBS and 10% DMSO) for cryopreservation of isolated human mature adipocytes from adipose tissue in -80 degree. When I freezed the cells, the number of cell was...
26 May 2024 7,802 0 View
Hi, I have been working with 3T3-L1 cells for over a year now.However, over the last couple of months they stopped differentiating into adipocytes. I seed at 200k cells/well (12 well plate), and...
23 May 2024 3,356 1 View
I am evaluating histological slides (H&E) of brown adipose tissue from rats. And counting the number of unilocular adipocytes to evaluate the whitening process. How to identify? Would it...
13 May 2024 7,876 0 View
As human adipocytes can be up to 300 μm in diameter, How these cells can be analyzed for cell markers by flow cytometry with 100 micron Nozzles? What changes are necessary to be done on flow...
12 May 2024 2,253 0 View
Going futher, i am runing a nutrition experiment using black soldier fly meal (BSFM) and polychaete meal (PM) as a replacement for fishmeal targeting maturation in Litopenaeus vannamei. This is...
11 May 2024 3,985 0 View
Please i need a proper protocol to isolate intramuscular preadipocyte from the rabbit
16 April 2024 9,395 0 View