I have to determine adipose tissue stiffness. My samples were preserved in NBF. Can I use those tissue sections? or should I use fresh tissue to identify tissue stiffness? Is there any protocol that can help me?
After UVO treatment on PDMS, I got C=O stretch peak from ATR-FTIR. I used Sylgard184 (base:curing agent=100:4), and treated UVO 90 minutes on cured PDMS by UV ozone cleaner.
05 April 2024 1,902 0 View
I need to determine indentation and tissue stiffness. I already have paraffin embedded tissue, but not sure if that will give an accurate result or I should use a fresh tissue.
11 February 2024 2,114 0 View
I have to characterise fibroblasts and preadipocytes from fat tissue. MOst of the articles have used DMEM/ F12, while some have used DMEM. Is there any rational behind using specific media over...
21 November 2023 4,268 0 View
Examples of informal publishing: Blogs Podcasts Letters/Email YouTube videos Examples of formal publishing: Peer-reviewed journal articles Books printed by scholarly publishers Conference...
10 October 2023 3,434 0 View
I am sorting preadipocytes from SVF and now need to differentiate them into mature adipocytes. Does a mixed cocktail of preadipocyte differentiation media works best of making a cocktail by...
07 October 2023 1,142 0 View
I have to differenitate stem cells into preadipocyte and further into mature adipocyte. We want to quantify ECM produced by adipocytes. Not sure if I should use Immunoflurence or there is any...
04 September 2023 6,458 0 View
Given the disproportionately high risk provided by blood-borne pathogens, regular monitoring of blood transfusions is required to avoid disease transmission. ELISA and Rapid diagnostic...
01 September 2023 7,864 1 View
As I know, there are 3 reference energy to find or compare band level when we need to band alignment data 1. fermi energy set to zero 2. Vacuum energy (from slab calculation) set to zero 3. Align...
03 August 2023 2,186 0 View
What's the hallmark of adipose tissue fibrosis? What techniques can be performed to identify and quantify adipose tissue fibrosis?
24 July 2023 2,704 0 View
I have to characterize fibroblast and myofibroblast in adipose tissue. Since these are connective tissue cells and ubiquitously present in all connective tissue therefore, isolation from a...
18 June 2023 1,307 1 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,114 1 View
We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...
08 August 2024 4,841 0 View
I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...
07 August 2024 1,027 0 View
I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...
07 August 2024 1,685 1 View
Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...
07 August 2024 9,561 0 View
Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...
07 August 2024 3,217 4 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,365 3 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 500 2 View