I need to determine indentation and tissue stiffness.
I already have paraffin embedded tissue, but not sure if that will give an accurate result or I should use a fresh tissue.
After UVO treatment on PDMS, I got C=O stretch peak from ATR-FTIR. I used Sylgard184 (base:curing agent=100:4), and treated UVO 90 minutes on cured PDMS by UV ozone cleaner.
05 April 2024 1,902 0 View
I have to determine adipose tissue stiffness. My samples were preserved in NBF. Can I use those tissue sections? or should I use fresh tissue to identify tissue stiffness? Is there any protocol...
01 February 2024 7,883 0 View
I have to characterise fibroblasts and preadipocytes from fat tissue. MOst of the articles have used DMEM/ F12, while some have used DMEM. Is there any rational behind using specific media over...
21 November 2023 4,268 0 View
Examples of informal publishing: Blogs Podcasts Letters/Email YouTube videos Examples of formal publishing: Peer-reviewed journal articles Books printed by scholarly publishers Conference...
10 October 2023 3,434 0 View
I am sorting preadipocytes from SVF and now need to differentiate them into mature adipocytes. Does a mixed cocktail of preadipocyte differentiation media works best of making a cocktail by...
07 October 2023 1,142 0 View
I have to differenitate stem cells into preadipocyte and further into mature adipocyte. We want to quantify ECM produced by adipocytes. Not sure if I should use Immunoflurence or there is any...
04 September 2023 6,458 0 View
Given the disproportionately high risk provided by blood-borne pathogens, regular monitoring of blood transfusions is required to avoid disease transmission. ELISA and Rapid diagnostic...
01 September 2023 7,864 1 View
As I know, there are 3 reference energy to find or compare band level when we need to band alignment data 1. fermi energy set to zero 2. Vacuum energy (from slab calculation) set to zero 3. Align...
03 August 2023 2,186 0 View
What's the hallmark of adipose tissue fibrosis? What techniques can be performed to identify and quantify adipose tissue fibrosis?
24 July 2023 2,704 0 View
I have to characterize fibroblast and myofibroblast in adipose tissue. Since these are connective tissue cells and ubiquitously present in all connective tissue therefore, isolation from a...
18 June 2023 1,307 1 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 500 2 View
I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...
03 August 2024 9,917 0 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 588 4 View
I am planning to prepare a decellularized porcine tissue derived biomaterial. It would be very helpful if I get to know the composition of the solution which is ideal to bring the tissue sample...
31 July 2024 5,645 2 View
I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...
29 July 2024 3,030 2 View
My question pertaining to the DAB staining in cytoplasm of human oral squamous cell carcinoma tissue. When quantifying the epithelial cancer cells do we have to crop remove the stromal tissue?...
29 July 2024 2,682 6 View
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24 July 2024 1,558 1 View
Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...
23 July 2024 2,844 1 View
This is a slice of intestinal tissue from the Chinese mitten crab (intestine in the upper right corner). I injected WSSV into this horseshoe crab. Now I want to know what these red spots next to...
18 July 2024 5,602 0 View