I have read that SSR primer is locus specific which I understand as there is only one SSR amplicon from a single chromosome. or does region to which SSR primers bind to,are they present more than once in a chromosome.Please clarify.
The total simulation time and time steps are 5000 fs and 1 fs, respectively. The OSZICAR file seems incomplete and it gives the energy in only two steps. I have attached my INCAR and OSZICAR.out...
24 April 2024 1,149 0 View
I would like to know why the peak anodic current during the oxidation of Cu is present in the CV of a microelectrode during copper dissolution. Au microelectrode (30um) was the working electrode....
12 June 2023 9,814 5 View
I am using Cu in Copper sulphate solution as a reference electrode. I would like to know how this reference electrode maintains potential during electrodeposition.
28 April 2023 8,389 1 View
The permitted range of element concentrations in cowpea seeds using ICPMS can be found in the literature. However, there is no such range in the case of seedlings. Please let me know the range of...
27 February 2022 7,557 2 View
In most of the perovskite oxides, the conduction mechanism is constituted by the hopping of charge carriers. so what is the physical significance of ρ_0 in the VRH mechanism
04 January 2022 3,232 1 View
I'm working on polyploid crop with intraspecific ploidy variation. Our crop do not have a reference genome. I need to find SNP's associated with traits and for that intend to use ddRAD...
09 May 2021 5,132 4 View
Recently we have installed Gamma spectrometry set up at our institution. It has NaI flat type detector of size 3"x3". To analyze soil, sand, rock, and other environmental samples we require...
29 March 2021 971 1 View
Is it possible to develop DNA fingerprinting for varietal identification of crop plants without reference genome and no locus information except the availability of set of DNA markers.
25 January 2021 7,113 4 View
I 'am looking for a most simplified formula to quantify shape of leaves mathematically which will help me to categorise plants based on leaf shape.Earlier,I tried with some image processing...
10 December 2020 2,574 5 View
Is it possible to to fertilize the eggs of rainbow trout after 3-4 hours of spawning? If yes, what would be the procedures to keep safe for high rate of fertilization? How many hours we can hold...
30 October 2020 2,351 2 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...
30 July 2024 841 2 View
I want to introduce 2 mutations using Agilent's multi-site mutagenesis kit, I designed the primers using their online tool and it gave me 2 sets for primers (1 set for each mutation) so I was...
25 July 2024 6,517 3 View
Hi everyone, I have extracted DNA from a biogas bioreactor using Qiagen kit and prep cDNA library then used this library as template to optimize primers for qPCR (taken from papers). Some of the...
23 July 2024 1,329 5 View