I have read that SSR primer is locus specific which I understand as there is only one SSR amplicon from a single chromosome. or does region to which SSR primers bind to,are they present more than once in a chromosome.Please clarify.
Is it possible to develop DNA fingerprinting for varietal identification of crop plants without reference genome and no locus information except the availability of set of DNA markers.
25 January 2021 6,826 4 View
I 'am looking for a most simplified formula to quantify shape of leaves mathematically which will help me to categorise plants based on leaf shape.Earlier,I tried with some image processing...
10 December 2020 2,386 5 View
If I select polygon samples to train my model, how the value of pixels used? Does the model take DN of each pixel or the average of each object (polygon)?
01 October 2020 4,882 2 View
I need to send few powdered leaf samples crushed using liquid nitrogen for flow cytometric analysis.Will the DNA get damaged if it's not packed in ice?
19 June 2020 7,259 6 View
Hi, I have trouble in understanding GWAS research papers (as they are full of statistics... )for finding phenotypic to genotypic relationship in crop plants.Can someone suggest me the basic...
15 January 2020 4,914 2 View
The genomic DNA,I isolated from turmeric using ctab .The protocol followed for genomicDNA isolation is: grind turmeric leaves using DNA extraction buffer(100 mM tris PH 8,20 mM EDTA,1.4 M Nacl,2%...
07 November 2019 9,428 10 View
when extracting stress values S33 at mid section of composite girder analysed in ABAQUS, getting both negative and positive values at 5 - 6 nodes nearer to the interface. this indicates like...
06 June 2019 7,901 2 View
I am doing Msc project,so my Msc project I want to ask this question. Nano particles are not soluble in most of the solvent
13 February 2019 4,312 8 View
I was reading about the relation between Kurtosis and Outliers on Wiki and got across a line 'An example of a platykurtic distribution is the uniform distribution, which does not produce outliers'...
02 August 2018 8,845 3 View
Hi,I am trying to find variation in capacitance between two plates with applied body load. I defined an air box around the plates. Now I'm trying with electromechanics physics for applying body...
13 April 2016 1,935 1 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...
03 March 2021 8,066 4 View
So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...
02 March 2021 1,146 2 View
I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...
01 March 2021 360 7 View
I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...
28 February 2021 606 3 View
hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?
28 February 2021 1,254 3 View
Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.
28 February 2021 5,008 4 View
Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...
26 February 2021 5,029 2 View
Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...
25 February 2021 6,969 3 View
Hi all, I am doing a genescan analysis for a deleted exon in the patient sample. The amplified region is 215 bp. In the patient sample because of homozygous deletion, no peak is observed while in...
23 February 2021 5,645 1 View