Aswathi A.P

9 Questions 14 Answers 0 Followers

Questions related from Aswathi A.P

Can someone help me with ddRAD in polyploid crops without reference genome?

I'm working on polyploid crop with intraspecific ploidy variation. Our crop do not have a reference genome. I need to find SNP's associated with traits and for that intend to use ddRAD...

10 May 2021 5,168 4 View

Can someone explain how to carry out DNA fingerprinting in crop plant without reference genome and no locus information?

Is it possible to develop DNA fingerprinting for varietal identification of crop plants without reference genome and no locus information except the availability of set of DNA markers.

26 January 2021 7,158 4 View

Can someone help me with analysing raw ddRAD sequence data ?

I would like to know about any single user-friendly software for ddRAD raw sequence data (Fastq format) without a reference genome.

24 December 2020 3,006 4 View

Can someone help me find a way to quantify leaf shape?

I 'am looking for a most simplified formula to quantify shape of leaves mathematically which will help me to categorise plants based on leaf shape.Earlier,I tried with some image processing...

11 December 2020 2,608 5 View

Is it possible to calculate Linkage disequilibrium for association mapping in polyploid crop plants in which allelic dosage calculation is difficult?

I 'm working on genetic diversity analysis of crop plants with variable ploidy level using SSR markers. I suppose calculation of heterozygous frequencies and allelic dosage etc would be difficult...

18 October 2020 7,244 4 View

How can we understand SSR alleles in relation to chromosomes in polyploid plants?

I have read that SSR primer is locus specific which I understand as there is only one SSR amplicon from a single chromosome. or does region to which SSR primers bind to,are they present more than...

28 June 2020 7,664 0 View

Can I send leaf samples crushed and powdered in liquid nitrogen for ploidy analysis without icepacks?

I need to send few powdered leaf samples crushed using liquid nitrogen for flow cytometric analysis.Will the DNA get damaged if it's not packed in ice?

20 June 2020 7,456 6 View

Can someone help me with Genome wide association study in crop plants to find marker trait association?

Hi, I have trouble in understanding GWAS research papers (as they are full of statistics... )for finding phenotypic to genotypic relationship in crop plants.Can someone suggest me the basic...

16 January 2020 5,099 2 View

Can anyone help me with Genomic DNA isolation from curcuma longa using ctab method?

The genomic DNA,I isolated from turmeric using ctab .The protocol followed for genomicDNA isolation is: grind turmeric leaves using DNA extraction buffer(100 mM tris PH 8,20 mM EDTA,1.4 M Nacl,2%...

08 November 2019 9,640 10 View