How many hours can keep the eggs of rainbow trout viable without fertilization (without mixing the milt)?

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What is the reason Bax protein size is small than it should be in Western blot?

I am trying to express Bax using PMIG- Bax plasmid from add gene. When I express it in Mouse Embryonic Fibroblast using retroviral transduction I am getting protein at around 13/14 kDA instead of...

21 April 2023 3,003 3 View

I want to find out the porosity of the sponge?As I followed this protocol which I have explained in description?

The porosity of sponges was measured by the solvent method. The solvent was chosen as ethanol because of the insolubility of sponges in ethanol. A certain volume of the sponge was cut accurately....

14 August 2022 5,745 0 View

Has anyone tried extracting DNA from potato roots using DNeasy plant pro DNA kit?

Need some advice

24 June 2021 2,650 5 View

Can someone help me with ddRAD in polyploid crops without reference genome?

I'm working on polyploid crop with intraspecific ploidy variation. Our crop do not have a reference genome. I need to find SNP's associated with traits and for that intend to use ddRAD...

09 May 2021 5,132 4 View

Who are the vendors for Marinelle Beakers in india for NaI flatype (3"x3") type detector for gamnma spectrometry?

Recently we have installed Gamma spectrometry set up at our institution. It has NaI flat type detector of size 3"x3". To analyze soil, sand, rock, and other environmental samples we require...

29 March 2021 971 1 View

Can someone explain how to carry out DNA fingerprinting in crop plant without reference genome and no locus information?

Is it possible to develop DNA fingerprinting for varietal identification of crop plants without reference genome and no locus information except the availability of set of DNA markers.

25 January 2021 7,113 4 View

Can some body help me to figure out which General Climatic Model is suitable in case of Asia particularly Bhutan?

There are total of nine GCM from CIMP 6, and I am confused which model suits best for my study . I want to determine change in land suitibility with respect to 3 SSP, RCP 2.6, 4.5 And 8.5 for...

25 December 2020 8,797 2 View

Can someone help me find a way to quantify leaf shape?

I 'am looking for a most simplified formula to quantify shape of leaves mathematically which will help me to categorise plants based on leaf shape.Earlier,I tried with some image processing...

10 December 2020 2,574 5 View

How can I run movestay command?

I used the following command: movestay (lnTotalincome = HHsize h1sex TLU yrcop tland tprod) (ccert = HHsize h1sex TLU yrcop tland tprod), select (train = h1edu) But this doesnot work and I get...

01 November 2020 1,367 2 View

Is it possible to calculate Linkage disequilibrium for association mapping in polyploid crop plants in which allelic dosage calculation is difficult?

I 'm working on genetic diversity analysis of crop plants with variable ploidy level using SSR markers. I suppose calculation of heterozygous frequencies and allelic dosage etc would be difficult...

17 October 2020 7,208 4 View

How do I replace a file with a more recent version of a paper that was uploaded to ResearchGate?

The paper in question is "Interpolation of Nitrogen Fertilizer Use in Canada from Fertilizer Use Surveys". This paper was very recently published by Agronomy (MDPI). Agronomy has, in the last day...

07 August 2024 9,934 3 View

Does egg white interfere with any histology staining?

I have mouse lung tissue which is mounted with egg whites.I need to stain my tissues with PAS and HE. Can egg whites interfere with these stainings?

02 July 2024 8,261 0 View

What can cause that mosquitoes from Rockefeller reference strain (Aedes aegypti) spend too much time in larval stages?

We have been rearing Rockefeller mosquitoes for many years in our laboratory without any problems, but lately, in spite of good viability of eggs and unchanged environmental conditions (27°C,...

20 June 2024 3,515 2 View

Effect overheating egg on nutritional value and man's health ?

Effect of heating and nutritional value loss

17 June 2024 2,159 1 View

What concentration is suitable for foliar application of nitrogen fertilizers?

Urea fertilizer concentration percentage of nitrogen

07 June 2024 488 5 View

What is the hemagglutination assay for fiber protein of egg drop syndrome virus(EDSV) ?

How can I use EDSV fiber protein antigen for hemagglutination test?

05 June 2024 1,459 0 View

How top dressing of nitrogenous fertilizers can be done in mulched wheat and rice crops and how should FYM/compost be applied in zero till wheat?

02 June 2024 3,836 3 View

Fish eggs ID?

Any idea of the species? Catch in may, near roscoff in brittany, No oil globule, size 1.2mm The specimen is well marked with melanophore, and pectoral fins are already pigmented (as seen on...

26 May 2024 7,624 0 View

What are the advantages of organic fertilization machines?

organic fertilization machines

16 May 2024 1,376 1 View

The best media for IVP in mice?

Hello community! What is for you the best combination of media for IVM, IVF and CIV in mice? In my experience we usually use media made in our laboratory but I have the opportunity to purchase...

02 May 2024 2,548 0 View