How many hours can keep the eggs of rainbow trout viable without fertilization (without mixing the milt)?

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Can someone explain how to carry out DNA fingerprinting in crop plant without reference genome and no locus information?

Is it possible to develop DNA fingerprinting for varietal identification of crop plants without reference genome and no locus information except the availability of set of DNA markers.

25 January 2021 6,826 4 View

Can some body help me to figure out which General Climatic Model is suitable in case of Asia particularly Bhutan?

There are total of nine GCM from CIMP 6, and I am confused which model suits best for my study . I want to determine change in land suitibility with respect to 3 SSP, RCP 2.6, 4.5 And 8.5 for...

25 December 2020 8,606 2 View

Can someone help me find a way to quantify leaf shape?

I 'am looking for a most simplified formula to quantify shape of leaves mathematically which will help me to categorise plants based on leaf shape.Earlier,I tried with some image processing...

10 December 2020 2,386 5 View

How can I run movestay command?

I used the following command: movestay (lnTotalincome = HHsize h1sex TLU yrcop tland tprod) (ccert = HHsize h1sex TLU yrcop tland tprod), select (train = h1edu) But this doesnot work and I get...

01 November 2020 1,065 2 View

In supervised classification, how polygon training sample works?

If I select polygon samples to train my model, how the value of pixels used? Does the model take DN of each pixel or the average of each object (polygon)?

01 October 2020 4,882 2 View

Has anyone tried electrospinning a polymer with higher concentration of salt (LiCl preferred)?

I am trying to electrospin polymer along with higher concentration of salt (more than 2% of salt). What might be the suitable polymer and suitable set up parameters?

30 July 2020 9,256 5 View

Can I send leaf samples crushed and powdered in liquid nitrogen for ploidy analysis without icepacks?

I need to send few powdered leaf samples crushed using liquid nitrogen for flow cytometric analysis.Will the DNA get damaged if it's not packed in ice?

19 June 2020 7,259 6 View

Can someone help me with Genome wide association study in crop plants to find marker trait association?

Hi, I have trouble in understanding GWAS research papers (as they are full of statistics... )for finding phenotypic to genotypic relationship in crop plants.Can someone suggest me the basic...

15 January 2020 4,914 2 View

Is hydrodynamic theroy of similarity required for limited number of iteration which is found in ansys fluent student version?

we conducted our cfd analysis in ansys fluent in student version and directly kept the results after convergence? Is it necessary to apply hydrodynamcis theory of similarity on the results and...

11 November 2019 722 4 View

Can anyone help me with Genomic DNA isolation from curcuma longa using ctab method?

The genomic DNA,I isolated from turmeric using ctab .The protocol followed for genomicDNA isolation is: grind turmeric leaves using DNA extraction buffer(100 mM tris PH 8,20 mM EDTA,1.4 M Nacl,2%...

07 November 2019 9,428 10 View

Are these moths Spodoptera frugiperda?

The caterpillars of the moths were collected as fruitborers in pepper at a farmer. I reared them till the adults hatched and compared them with pictures and descriptions on the internet. Are these...

24 February 2021 7,785 5 View

Seeds harvest from white clover by selfing?

I tried to get seeds from white clover by self-fertilization but I could not get the seeds. As you know the white clover is generally fertilized by crossing. I wanna get the seeds by...

04 February 2021 9,152 4 View

Helicoverpa armigera eggs are not hatching in lab condition, why?

Dear RG friends, I am providing a complete H. armigera diet for larvae and maintaining the eggs in the growth chamber as well as in-room conditions. But since three months, eggs laid from the...

21 January 2021 5,546 32 View

Detection of insect eggs in stored and processed products?

Currently, in order to identify insect eggs in stored or processed products (such as contaminated flour or wafer samples), staining was performed based on the method mentioned in the below...

15 January 2021 6,362 5 View

Which test to use check normality for count data if I have 3 treatments and one dependent variable?

I am working on a experimental design. I have three treatments(three temperatures) . I have 18 fish under each sample and after a desired time I will check if there is any variation in their...

01 January 2021 9,167 3 View

Time taken from pollination to fertilization in solanaceous crops?

How much time is required from germination of pollen grain on stigma to fertilization in Solanaceous crops (chilli, tomato, brinjal)?

26 December 2020 887 5 View

What is the preferable incubation period in injecting ethanol into the duck eggs for antiteratogenic studies?

I have been working on the anti-teratogenic activity of a plant extract towards ethanol induced duck embryos however, many studies use the 14 days incubation period and then remove the embryo for...

17 November 2020 4,701 5 View

How can I get eggs of an univoltine casebearer moth for a threshold study?

I am planning to conduct a cage study to determine a critical density of an univoltine casebearer moth in red clover seed crop. This requires to release first instar larvae in the cages with the...

02 November 2020 6,577 3 View

How can I get help to identify a species of Coniopterygidae from Peru?

I found a species of Coniopterygidae preying on eggs and nymphs of Aleurothrixus floccosus in a lemon tree. I have followed its biology and have pictures of eggs, larvae, pupae and adults. With my...

16 October 2020 6,421 2 View

What is the protocol of eggs sampling from an eggs production unit ?

Dear researchers greetings, I'm working on eggs quality for my Ph.D thesis and I want to know what is the protocol for eggs sampling from a production unit. The main questions I have are the...

13 October 2020 9,716 7 View