Is it possible to develop DNA fingerprinting for varietal identification of crop plants without reference genome and no locus information except the availability of set of DNA markers.
This document was prepared with support from the Standing Panel on Impact Assessment (SPIA) of CGIAR and the GEMS agroinformatics initiative at the University of Minnesota (UMN). Poets was an agroinformatics analyst with GEMS and is presently genetics project lead at Syngenta, Slater, IA; Silverstein is scientific lead at the Minnesota Supercomputing Institute and operations lead of GEMS; Pardey is director of global research strategy for UMN’s College of Food, Agricultural and Natural Resource Sciences (CFANS) and co-director of GEMS; Hearne is a Principal Scientist at the International Maize and Wheat Improvement Center (CIMMYT); and Stevenson is a senior research fellow at the CGIAR Standing Panel on Impact Assessment. This paper solely reflects the opinions and findings of the authors.The content of this report benefited greatly from discussions with participants at the meeting “Scaling Best Practice on Integrating DNA Fingerprinting of Crops into Large-Scale Household Surveys”, convened by the CGIAR Standing Panel on Impact Assessment (SPIA) and the University of Minnesota and hosted by the Bill and Melinda Gates Foundation in Seattle, WA, on January 18-19, 2018. We are particularly grateful for detailed comments on prior drafts from Andrzej Kilian. We also thank Maxwell Mkondiwa, along with partici-pants in the Seattle workshop—especially Ismail Rabbi (IITA), Marianne Banziger (CIMMYT), Augusto Becerra (CIAT), and Mywish Maredia (Michigan State University)—for valuable input in the preparation of this report, and Heidi Fritschel for outstanding editorial revisions to the manuscript.