Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Please. Could you help be in implementing Fractal Discrete Cosine Transform algorithm in Matlab?

I do need the Matlab code of Fractal Discrete Cosine Transform (FDCT). Can anyone who has already implement this code, help me with the implementation of this transform?

24 February 2021 5,602 2 View

What is the reason film thickness increased?

what is the reason film thickness increased when increased doping concentrations give some reasons The measured film thickness is 156, 282, 394, 548 and 681 nm for 0, 1.5, 2.5, 3.5, and 4.5 wt....

17 February 2021 8,987 5 View

Which is the best wavelength to study aerosol optical depth(AOD) related to climate reseach,which criteria must be adopted in choosing the wavelengh?

We are doing a study on impact of aerosol on precipitation. Which satellite data and which wavelength will be the best. or can some one suggest a criteria to be adopted to choose an instrument...

12 February 2021 6,324 3 View

Queries regarding the rain flow counting algorithm?

02 February 2021 9,790 2 View

How do Research Gate proceed to integrate in the reference data publications and citations the recently published journals ?

How do Research Gate proceed to integrate in the reference data for publications and citations the recently published journals - for instance for the last months of 2020 and for instance Intl J...

02 February 2021 9,336 7 View

How to take XRD of colloidal Ag NPs?

I have synthesized silver nanoparticles using sodium citrate as reducing agent. I have carried out XRD characterization by drop coating colloidal solution on the glass substrate ( since yield in...

27 January 2021 1,416 3 View

Can someone explain how to carry out DNA fingerprinting in crop plant without reference genome and no locus information?

Is it possible to develop DNA fingerprinting for varietal identification of crop plants without reference genome and no locus information except the availability of set of DNA markers.

25 January 2021 6,826 4 View

How to revive bacteria from glycerol stock?

19 January 2021 7,025 4 View

How to determine the genetic potential of the genotype in terms of fruit size and weight?

In addition to genetic and environmental factors, fruit size is also influenced by crop load, rootstock, orchard planting system/tree training system, pollination, water and nutrient management,...

13 January 2021 9,309 4 View

Does anybody know the ways of homogeneous ripening of Mung bean crop?

Some crops (Mung bean) are matured at different times i.e., harvested at several times that's why the labour cost of the farmer is high. So, could you please, is there any ways or chemicals are...

12 February 2021 294 4 View

How to find at which time point there is significance and between which groups in a repeated measures ANOVA?

I have conducted a repeated-measures ANOVA and a Tukey post hoc test. The data I have is for 3 seedling mutant lines, I am comparing their root growth over a timecourse of 19 hours. Within each...

13 December 2020 9,868 5 View

Why do the allelopathic produce a positive effect?

Following an experiment where Zea mays and Cucurbita pepo seeds were planted individually and together, results showed a positive allelopathic effect on germination and seeling lenght as both...

03 December 2020 1,875 6 View

Why whitefly prefer not to infest Pigeonpea?

Whitefly, Bemisia tabaci is pest of broad host range. In pulse crops, it generally infests greengram, blackgram and cowpea but not Pigeonpea. There might be some biochemical or morphological...

09 November 2020 9,199 3 View

How can I convert microscopic image length in pixel to um for an image taken from Leica DM2500?

I have been doing work on seedling stomata. the old images cannot be measured in um but can in pixel. so I need to convert the measurement to um.

30 September 2020 7,979 4 View

Can anyone help me with quality of seed roots in this picture?

So I grew some radish seeds in a petri-dish using two different biochars. Is it correct to conclude that the seed number 2 is healthier than number 1? Can anybody help me to understand the growth...

12 March 2020 6,645 5 View

Can I use soft vacuum filtration to harvest Ath seedlings from liquid media?

Hello, I'm growing different accessions of Arabidopsis thaliana and so far we have been harvesting them by pouring the media over a simple sieve, then putting them on paper towels for a minute...

18 February 2020 7,944 3 View

Is any research paper available on bioinsecticidal properties of some plant extracts on Cowpea weevil (Callosobruchus maculatus)?

We want to see the bioinsecticidal properties of some plant extracts on Cowpea weevil (Callosobruchus maculatus) on stored Cowpea Vigna unguiculata.

13 February 2020 8,987 8 View

Is there a working protocol for microtomy of Vigna radiata root samples?

A simple and cost-effective protocol for sectioning of Vigna radiata root samples.

02 February 2020 786 1 View

Can I use soft vacuum filtration to harvest Ath seedlings from liquid media?

Hello, I'm growing different accessions of Arabidopsis thaliana and so far we have been harvesting them by pouring the media over a simple sieve, then putting them on paper towels for a minute...

01 February 2020 3,282 2 View